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Literature summary extracted from

  • Pandey, S.; Shrivastava, A.K.; Singh, V.K.; Rai, R.; Singh, P.K.; Rai, S.; Rai, L.C.
    A new arsenate reductase involved in arsenic detoxification in Anabaena sp. PCC7120 (2013), Funct. Integr. Genomics, 13, 43-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.1
-
 Nostoc sp.
1.20.4.1 expression in Escherichia coli Nostoc sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.20.4.1 comparative homology modeling. Along with Cys11, the residues like Ile8, Pro9, Asn10, Gly12, Thr13, Cys14, Lys15, and Phe18 also show coordination with arsenate Nostoc sp.
1.20.4.1 molecular modeling and docking with asenate oxyanion. Residues Cys11, Ile8, Pro9, Asn10, Gly12, Thr13, Cys14, Lys15, and Phe18 show coordination with arsenate Nostoc sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.1 13624
-
 x * 13624, calculated Nostoc sp.

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.1 Nostoc sp. Q8Z0A3
-
-
1.20.4.1 Nostoc sp. PCC 7120 Q8Z0A3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.1 arsenate + reduced glutaredoxin
-
 Nostoc sp. arsenite + oxidized glutaredoxin
-
 ?
1.20.4.1 arsenate + reduced glutaredoxin enzyme catalyzes the oxidation of NADPH coupled with reduction of arsenate in the presence of glutathione reductase, glutathione and glutaredoxin Nostoc sp. arsenite + oxidized glutaredoxin
-
 ?
1.20.4.1 arsenate + reduced glutaredoxin
-
 Nostoc sp. PCC 7120 arsenite + oxidized glutaredoxin
-
 ?
1.20.4.1 arsenate + reduced glutaredoxin enzyme catalyzes the oxidation of NADPH coupled with reduction of arsenate in the presence of glutathione reductase, glutathione and glutaredoxin Nostoc sp. PCC 7120 arsenite + oxidized glutaredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.4.1 ? x * 13624, calculated Nostoc sp.

Synonyms

EC Number Synonyms Comment Organism
1.20.4.1 All0195
-
 Nostoc sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.20.4.1 65
-
 stable up to Nostoc sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.20.4.1 Nostoc sp. calculated
-
 8.9

Expression

EC Number Organism Comment Expression
1.20.4.1 Nostoc sp. enhanced expression under sodium arsenate stress up

General Information

EC Number General Information Comment Organism
1.20.4.1 physiological function enzyme complements the arsenate-sensitive phenotype of Escherichia coli Nostoc sp.
1.20.4.1 physiological function protein functionally complements the arsenate reductase-deficient sodium arsenate-hypersensitive phenotype of Escherichia coli strain WC3110 Nostoc sp.