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Literature summary extracted from

  • Wei, J.; Timler, J.G.; Knutson, C.M.; Barney, B.M.
    Branched-chain 2-keto acid decarboxylases derived from Psychrobacter (2013), FEMS Microbiol. Lett., 346, 105-112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.72 expressed in Escherichia coli BL21 cells Lactococcus lactis
4.1.1.72 expressed in Escherichia coli BL21 cells Psychrobacter cryohalolentis
4.1.1.72 expressed in Escherichia coli BL21 cells Psychrobacter arcticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.72 55000
-
x * 55000, SDS-PAGE Lactococcus lactis
4.1.1.72 55000
-
x * 55000, SDS-PAGE Psychrobacter cryohalolentis
4.1.1.72 55000
-
x * 55000, SDS-PAGE Psychrobacter arcticus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.72 Lactococcus lactis
-
-
-
4.1.1.72 Psychrobacter arcticus
-
-
-
4.1.1.72 Psychrobacter arcticus 273-4
-
-
-
4.1.1.72 Psychrobacter cryohalolentis
-
-
-
4.1.1.72 Psychrobacter cryohalolentis K5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.72 nickel-charged metal chelating column chromatography Lactococcus lactis
4.1.1.72 nickel-charged metal chelating column chromatography Psychrobacter cryohalolentis
4.1.1.72 nickel-charged metal chelating column chromatography Psychrobacter arcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.72 3-methyl-2-oxobutyrate
-
Lactococcus lactis 3-methylpropanal + CO2
-
?
4.1.1.72 3-methyl-2-oxobutyrate
-
Psychrobacter cryohalolentis 3-methylpropanal + CO2
-
?
4.1.1.72 3-methyl-2-oxobutyrate
-
Psychrobacter arcticus 3-methylpropanal + CO2
-
?
4.1.1.72 3-methyl-2-oxobutyrate
-
Psychrobacter cryohalolentis K5 3-methylpropanal + CO2
-
?
4.1.1.72 3-methyl-2-oxobutyrate
-
Psychrobacter arcticus 273-4 3-methylpropanal + CO2
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Lactococcus lactis 2-methylbutanal + CO2
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis 2-methylbutanal + CO2
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Psychrobacter arcticus 2-methylbutanal + CO2
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis K5 2-methylbutanal + CO2
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Psychrobacter arcticus 273-4 2-methylbutanal + CO2
-
?
4.1.1.72 4-methyl-2-oxopentanoate
-
Lactococcus lactis 4-methylbutanal + CO2
-
?
4.1.1.72 4-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis 4-methylbutanal + CO2
-
?
4.1.1.72 4-methyl-2-oxopentanoate
-
Psychrobacter arcticus 4-methylbutanal + CO2
-
?
4.1.1.72 4-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis K5 4-methylbutanal + CO2
-
?
4.1.1.72 4-methyl-2-oxopentanoate
-
Psychrobacter arcticus 273-4 4-methylbutanal + CO2
-
?
4.1.1.72 Phenylpyruvate
-
Lactococcus lactis Phenylacetaldehyde + CO2
-
?
4.1.1.72 Phenylpyruvate
-
Psychrobacter cryohalolentis Phenylacetaldehyde + CO2
-
?
4.1.1.72 Phenylpyruvate
-
Psychrobacter arcticus Phenylacetaldehyde + CO2
-
?
4.1.1.72 Phenylpyruvate
-
Psychrobacter cryohalolentis K5 Phenylacetaldehyde + CO2
-
?
4.1.1.72 Phenylpyruvate
-
Psychrobacter arcticus 273-4 Phenylacetaldehyde + CO2
-
?
4.1.1.72 pyruvate
-
Lactococcus lactis ethanal + CO2
-
?
4.1.1.72 pyruvate
-
Psychrobacter cryohalolentis ethanal + CO2
-
?
4.1.1.72 pyruvate
-
Psychrobacter arcticus ethanal + CO2
-
?
4.1.1.72 pyruvate
-
Psychrobacter cryohalolentis K5 ethanal + CO2
-
?
4.1.1.72 pyruvate
-
Psychrobacter arcticus 273-4 ethanal + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.72 ? x * 55000, SDS-PAGE Lactococcus lactis
4.1.1.72 ? x * 55000, SDS-PAGE Psychrobacter cryohalolentis
4.1.1.72 ? x * 55000, SDS-PAGE Psychrobacter arcticus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.72 branched-chain 2-keto acid decarboxylase
-
Lactococcus lactis
4.1.1.72 branched-chain 2-keto acid decarboxylase
-
Psychrobacter cryohalolentis
4.1.1.72 branched-chain 2-keto acid decarboxylase
-
Psychrobacter arcticus
4.1.1.72 KDC
-
Psychrobacter cryohalolentis
4.1.1.72 KDC
-
Psychrobacter arcticus
4.1.1.72 KdcA
-
Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.72 30
-
-
Psychrobacter arcticus
4.1.1.72 35
-
-
Psychrobacter cryohalolentis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.72 1.7
-
phenylpyruvate pH and temperature not specified in the publication Psychrobacter arcticus
4.1.1.72 1.8
-
phenylpyruvate pH and temperature not specified in the publication Psychrobacter cryohalolentis
4.1.1.72 7
-
4-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter arcticus
4.1.1.72 8.5
-
4-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter cryohalolentis
4.1.1.72 23
-
3-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter arcticus
4.1.1.72 25
-
3-methyl-2-oxopentanoate pH and temperature not specified in the publication Lactococcus lactis
4.1.1.72 27
-
3-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter cryohalolentis
4.1.1.72 29
-
3-methyl-2-oxobutyrate pH and temperature not specified in the publication Psychrobacter arcticus
4.1.1.72 33
-
3-methyl-2-oxobutyrate pH and temperature not specified in the publication Psychrobacter cryohalolentis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.72 6 7
-
Lactococcus lactis
4.1.1.72 6 7
-
Psychrobacter cryohalolentis
4.1.1.72 6 7
-
Psychrobacter arcticus