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Literature summary extracted from

  • Yoshida, H.; Yoshihara, A.; Teraoka, M.; Yamashita, S.; Izumori, K.; Kamitori, S.
    Structure of L-rhamnose isomerase in complex with L-rhamnopyranose demonstrates the sugar-ring opening mechanism and the role of a substrate sub-binding site (2013), FEBS Open Bio, 3, 35-40.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.14 mutant enzyme H101N in complex with L-rhamnose and D-allose, vapor diffusion method, using 7-8% (w/v) polyethylene glycol 20000 and 50 mM MES buffer (pH 6.3) Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.14 D327N inactive Pseudomonas stutzeri
5.3.1.14 H101N the enzymatic activity of the mutant is decreased Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 0.92
-
 L-rhamnose wild type enzyme, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 2.7
-
 D-allose wild type enzyme, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 9.4
-
 L-rhamnose mutant enzyme H101N, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 60
-
 D-allose mutant enzyme H101N, pH and temperature not specified in the publication Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.14 L-Rhamnose Pseudomonas stutzeri
-
 L-Rhamnulose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Pseudomonas stutzeri Q75WH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Pseudomonas stutzeri D-psicose
-
 r
5.3.1.14 L-Rhamnose
-
 Pseudomonas stutzeri L-Rhamnulose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-RhI
-
 Pseudomonas stutzeri
5.3.1.14 Rhamnose isomerase
-
 Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 16.8
-
 D-allose wild type enzyme, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 24.5
-
 D-allose mutant enzyme H101N, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 192
-
 L-rhamnose mutant enzyme H101N, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 374
-
 L-rhamnose wild type enzyme, pH and temperature not specified in the publication Pseudomonas stutzeri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.41
-
 D-allose mutant enzyme H101N, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 6.2
-
 D-allose wild type enzyme, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 20
-
 L-rhamnose mutant enzyme H101N, pH and temperature not specified in the publication Pseudomonas stutzeri
5.3.1.14 410
-
 L-rhamnose wild type enzyme, pH and temperature not specified in the publication Pseudomonas stutzeri