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Literature summary extracted from

  • Umehara, T.; Kim, J.; Lee, S.; Guo, L.T.; Söll, D.; Park, H.S.
    N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo (2012), FEBS Lett., 586, 729-733.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.26 L301M/Y306L/C348S/A315V while the wild type enzyme has a negligible charging activity for N-acetyl lysine, the mutant enzyme is able to acylate only N-acetyl lysine (not natural amino acids) onto tRNAPyl Methanosarcina mazei
6.1.1.26 L301M/Y306L/L309A/C348F while the wild type enzyme has a negligible charging activity for N-acetyl lysine, the mutant enzyme is able to acylate only N-acetyl lysine (not natural amino acids) onto tRNAPyl Methanosarcina mazei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.26 7.8
-
 N-acetyl-L-lysine pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/C348S/A315V Methanosarcina mazei
6.1.1.26 35.3
-
 N-acetyl-L-lysine pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/L309A/C348F Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl Methanosarcina mazei
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl Methanosarcina mazei DSM 3647
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.26 Methanosarcina mazei Q8PWY1
-
-
6.1.1.26 Methanosarcina mazei DSM 3647 Q8PWY1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl Methanosarcina mazei AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina mazei DSM 3647 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl Methanosarcina mazei DSM 3647 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + N-acetyl-L-lysine + tRNAPyl while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl Methanosarcina mazei AMP + diphosphate + N-acetyl-L-lysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + N-acetyl-L-lysine + tRNAPyl while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl Methanosarcina mazei DSM 3647 AMP + diphosphate + N-acetyl-L-lysyl-tRNAPyl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.26 PylRS
-
 Methanosarcina mazei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.26 0.00731
-
 N-acetyl-L-lysine pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/C348S/A315V Methanosarcina mazei
6.1.1.26 0.0323
-
 N-acetyl-L-lysine pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/L309A/C348F Methanosarcina mazei