EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.26 | L301M/Y306L/C348S/A315V | while the wild type enzyme has a negligible charging activity for N-acetyl lysine, the mutant enzyme is able to acylate only N-acetyl lysine (not natural amino acids) onto tRNAPyl | Methanosarcina mazei |
6.1.1.26 | L301M/Y306L/L309A/C348F | while the wild type enzyme has a negligible charging activity for N-acetyl lysine, the mutant enzyme is able to acylate only N-acetyl lysine (not natural amino acids) onto tRNAPyl | Methanosarcina mazei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.26 | 7.8 | - |
N-acetyl-L-lysine | pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/C348S/A315V | Methanosarcina mazei | |
6.1.1.26 | 35.3 | - |
N-acetyl-L-lysine | pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/L309A/C348F | Methanosarcina mazei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.26 | ATP + L-pyrrolysine + tRNAPyl | Methanosarcina mazei | - |
AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
6.1.1.26 | ATP + L-pyrrolysine + tRNAPyl | Methanosarcina mazei DSM 3647 | - |
AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.26 | Methanosarcina mazei | Q8PWY1 | - |
- |
6.1.1.26 | Methanosarcina mazei DSM 3647 | Q8PWY1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.26 | ATP + L-pyrrolysine + tRNAPyl | - |
Methanosarcina mazei | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
6.1.1.26 | ATP + L-pyrrolysine + tRNAPyl | while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl | Methanosarcina mazei | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
6.1.1.26 | ATP + L-pyrrolysine + tRNAPyl | - |
Methanosarcina mazei DSM 3647 | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
6.1.1.26 | ATP + L-pyrrolysine + tRNAPyl | while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl | Methanosarcina mazei DSM 3647 | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
6.1.1.26 | ATP + N-acetyl-L-lysine + tRNAPyl | while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl | Methanosarcina mazei | AMP + diphosphate + N-acetyl-L-lysyl-tRNAPyl | - |
? | |
6.1.1.26 | ATP + N-acetyl-L-lysine + tRNAPyl | while the wild type enzyme has a negligible charging activity for N-acetyl-L-lysine, the mutant enzyme is able to acylate only N-acetyl-L-lysine (not natural amino acids) onto tRNAPyl | Methanosarcina mazei DSM 3647 | AMP + diphosphate + N-acetyl-L-lysyl-tRNAPyl | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.26 | PylRS | - |
Methanosarcina mazei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.26 | 0.00731 | - |
N-acetyl-L-lysine | pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/C348S/A315V | Methanosarcina mazei | |
6.1.1.26 | 0.0323 | - |
N-acetyl-L-lysine | pH and temperature not specified in the publication, mutant enzyme L301M/Y306L/L309A/C348F | Methanosarcina mazei |