EC Number | Cloned (Comment) | Organism |
---|---|---|
6.5.1.1 | expression in Escherichia coli | Aeropyrum pernix |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.1 | KCl | 100 mM, approximately 70% inhibition | Aeropyrum pernix | |
6.5.1.1 | NaCl | 100 mM, approximately 70% inhibition | Aeropyrum pernix | |
6.5.1.1 | NH4Cl | 100 mM, approximately 70% inhibition | Aeropyrum pernix |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.1 | Ca2+ | the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix | |
6.5.1.1 | Co2+ | the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix | |
6.5.1.1 | KCl | optimum concentration: 5 mM | Aeropyrum pernix | |
6.5.1.1 | Mg2+ | optimal concentration: 15 mM, the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix | |
6.5.1.1 | Mn2+ | optimal concentration: 7.5 mM, the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.5.1.1 | 69000 | - |
1 * 69000, SDS-PAGE | Aeropyrum pernix |
6.5.1.1 | 69196 | - |
1 * 69196, the authors refer to the protein of 619 amino acids that is later reannotated as a protein of 602 amino acids (67747.6 Da), calculated from sequence | Aeropyrum pernix |
6.5.1.1 | 70000 | - |
gel filtration | Aeropyrum pernix |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.1 | Aeropyrum pernix | Q9YD18 | the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The UniProt-number refers to the 602 amino acid protein | - |
6.5.1.1 | Aeropyrum pernix DSM 11879 | Q9YD18 | the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The UniProt-number refers to the 602 amino acid protein | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.5.1.1 | - |
Aeropyrum pernix |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.1 | ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | activity with ADP is slightly lower than with ATP | Aeropyrum pernix | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
6.5.1.1 | ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | activity with ADP is slightly lower than with ATP | Aeropyrum pernix DSM 11879 | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
6.5.1.1 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme is inactive when ATP was substituted by AMP or NAD+ | Aeropyrum pernix | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
6.5.1.1 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme is inactive when ATP was substituted by AMP or NAD+ | Aeropyrum pernix DSM 11879 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.5.1.1 | monomer | 1 * 69000, SDS-PAGE | Aeropyrum pernix |
6.5.1.1 | monomer | 1 * 69196, the authors refer to the protein of 619 amino acids that is later reannotated as a protein of 602 amino acids (67747.6 Da), calculated from sequence | Aeropyrum pernix |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.5.1.1 | APE1094 | - |
Aeropyrum pernix |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.1 | 70 | - |
optimum for nick-closing activity is above 70°C | Aeropyrum pernix |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.1 | 65 | 80 | in the nick-closing assay, the recombinant ligase shows more than 80% activity in the temperature range 65-80°C. Ligase activity declines at temperatures below 35°C. A drastic decrease in activity is observed between 80 and 90°C | Aeropyrum pernix |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.1 | 100 | - |
stable for 60 min, half-life: 25 min | Aeropyrum pernix |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.1 | 7.5 | - |
optimum for nick-closing activity | Aeropyrum pernix |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.1 | 7 | 8.2 | 80% of maximal activity is observed between pH 7.0 and 8.2 | Aeropyrum pernix |