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Literature summary extracted from

  • Serrano, J.A.; Camacho, M.; Bonete, M.J.
    Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii (1998), FEBS Lett., 434, 13-16.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.1 dithiothreitol
-
 Haloferax volcanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 additional information reversible loss of activity in isocitrate lyase from Haloferax volcanii when the enzyme is out of extract without a thiol, such as dithiothreitol Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.00011
-
 glyoxylate pH 8.0, 40°C Haloferax volcanii
2.3.3.9 0.00012
-
 acetyl-CoA pH 8.0, 40°C Haloferax volcanii
4.1.3.1 1.16
-
 D-isocitrate pH 7.0, 40°C Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 KCl salt optimum: 3.0 M KCl Haloferax volcanii
2.3.3.9 Mg2+ activity is dependent on Mg2+ Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 67000
-
 3 * 67000, SDS-PAGE Haloferax volcanii
2.3.3.9 200000
-
 gel filtration Haloferax volcanii
4.1.3.1 47000
-
 3 * 47000, SDS-PAGE Haloferax volcanii
4.1.3.1 140000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.9 acetyl-CoA + H2O + glyoxylate Haloferax volcanii key enzyme of the glyoxylate cycle enzyme (S)-malate + CoA
-
 ?
2.3.3.9 acetyl-CoA + H2O + glyoxylate Haloferax volcanii DSM 3757 key enzyme of the glyoxylate cycle enzyme (S)-malate + CoA
-
 ?
4.1.3.1 isocitrate Haloferax volcanii key enzyme of the glyoxylate cycle succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate Haloferax volcanii DSM 3757 key enzyme of the glyoxylate cycle succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Haloferax volcanii
-
-
-
2.3.3.9 Haloferax volcanii DSM 3757
-
-
-
4.1.3.1 Haloferax volcanii
-
-
-
4.1.3.1 Haloferax volcanii DSM 3757
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Haloferax volcanii
4.1.3.1
-
 Haloferax volcanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.9 culture condition:acetate-grown cell no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is Haloferax volcanii
-
4.1.3.1 culture condition:acetate-grown cell no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is Haloferax volcanii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 acetyl-CoA + H2O + glyoxylate
-
 Haloferax volcanii (S)-malate + CoA
-
 ?
2.3.3.9 acetyl-CoA + H2O + glyoxylate key enzyme of the glyoxylate cycle enzyme Haloferax volcanii (S)-malate + CoA
-
 ?
2.3.3.9 acetyl-CoA + H2O + glyoxylate
-
 Haloferax volcanii DSM 3757 (S)-malate + CoA
-
 ?
2.3.3.9 acetyl-CoA + H2O + glyoxylate key enzyme of the glyoxylate cycle enzyme Haloferax volcanii DSM 3757 (S)-malate + CoA
-
 ?
4.1.3.1 D-isocitrate
-
 Haloferax volcanii succinate + glyoxylate
-
 ?
4.1.3.1 D-isocitrate
-
 Haloferax volcanii DSM 3757 succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate key enzyme of the glyoxylate cycle Haloferax volcanii succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate key enzyme of the glyoxylate cycle Haloferax volcanii DSM 3757 succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 homotrimer 3 * 67000, SDS-PAGE Haloferax volcanii
4.1.3.1 trimer 3 * 47000, SDS-PAGE Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.9 40
-
 assay at Haloferax volcanii
2.3.3.9 50 60
-
 Haloferax volcanii
4.1.3.1 40
-
 assay at Haloferax volcanii
4.1.3.1 55
-
-
 Haloferax volcanii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.9 25 80 25°C: about 45% of maximal activity, 80°C: about 45% of maximal activity Haloferax volcanii
4.1.3.1 40 60 40°C: 50% of maximal activity, 60°C: about 40% of maximal activity Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.9 8
-
 assay at Haloferax volcanii
2.3.3.9 8 9
-
 Haloferax volcanii
4.1.3.1 7
-
-
 Haloferax volcanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.9 7 10 pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity Haloferax volcanii
4.1.3.1 6.5 7.5 pH 6.5: about 70% of maximal activity, pH 7.5: about 55% of maximal activity Haloferax volcanii

General Information

EC Number General Information Comment Organism
2.3.3.9 physiological function key enzyme of the glyoxylate cycle enzyme Haloferax volcanii
4.1.3.1 physiological function key enzyme of the glyoxylate cycle Haloferax volcanii