BRENDA - Enzyme Database

Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii

Serrano, J.A.; Camacho, M.; Bonete, M.J.; FEBS Lett. 434, 13-16 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
dithiothreitol
-
Haloferax volcanii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
additional information
reversible loss of activity in isocitrate lyase from Haloferax volcanii when the enzyme is out of extract without a thiol, such as dithiothreitol
Haloferax volcanii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.3.9
0.00011
-
glyoxylate
pH 8.0, 40°C
Haloferax volcanii
2.3.3.9
0.00012
-
acetyl-CoA
pH 8.0, 40°C
Haloferax volcanii
4.1.3.1
1.16
-
D-isocitrate
pH 7.0, 40°C
Haloferax volcanii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.3.9
KCl
salt optimum: 3.0 M KCl
Haloferax volcanii
2.3.3.9
Mg2+
activity is dependent on Mg2+
Haloferax volcanii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.3.9
67000
-
3 * 67000, SDS-PAGE
Haloferax volcanii
2.3.3.9
200000
-
gel filtration
Haloferax volcanii
4.1.3.1
47000
-
3 * 47000, SDS-PAGE
Haloferax volcanii
4.1.3.1
140000
-
gel filtration
Haloferax volcanii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.3.9
acetyl-CoA + H2O + glyoxylate
Haloferax volcanii
key enzyme of the glyoxylate cycle enzyme
(S)-malate + CoA
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
Haloferax volcanii DSM 3757
key enzyme of the glyoxylate cycle enzyme
(S)-malate + CoA
-
-
?
4.1.3.1
isocitrate
Haloferax volcanii
key enzyme of the glyoxylate cycle
succinate + glyoxylate
-
-
?
4.1.3.1
isocitrate
Haloferax volcanii DSM 3757
key enzyme of the glyoxylate cycle
succinate + glyoxylate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.3.9
Haloferax volcanii
-
-
-
2.3.3.9
Haloferax volcanii DSM 3757
-
-
-
4.1.3.1
Haloferax volcanii
-
-
-
4.1.3.1
Haloferax volcanii DSM 3757
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.3.9
-
Haloferax volcanii
4.1.3.1
-
Haloferax volcanii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.3.9
culture condition:acetate-grown cell
no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is
Haloferax volcanii
-
4.1.3.1
culture condition:acetate-grown cell
no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is
Haloferax volcanii
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
727500
Haloferax volcanii
(S)-malate + CoA
-
-
-
-
2.3.3.9
acetyl-CoA + H2O + glyoxylate
key enzyme of the glyoxylate cycle enzyme
727500
Haloferax volcanii
(S)-malate + CoA
-
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
727500
Haloferax volcanii DSM 3757
(S)-malate + CoA
-
-
-
-
2.3.3.9
acetyl-CoA + H2O + glyoxylate
key enzyme of the glyoxylate cycle enzyme
727500
Haloferax volcanii DSM 3757
(S)-malate + CoA
-
-
-
?
4.1.3.1
D-isocitrate
-
727500
Haloferax volcanii
succinate + glyoxylate
-
-
-
?
4.1.3.1
D-isocitrate
-
727500
Haloferax volcanii DSM 3757
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
key enzyme of the glyoxylate cycle
727500
Haloferax volcanii
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
key enzyme of the glyoxylate cycle
727500
Haloferax volcanii DSM 3757
succinate + glyoxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.3.9
homotrimer
3 * 67000, SDS-PAGE
Haloferax volcanii
4.1.3.1
trimer
3 * 47000, SDS-PAGE
Haloferax volcanii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.3.9
40
-
assay at
Haloferax volcanii
2.3.3.9
50
60
-
Haloferax volcanii
4.1.3.1
40
-
assay at
Haloferax volcanii
4.1.3.1
55
-
-
Haloferax volcanii
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.3.9
25
80
25°C: about 45% of maximal activity, 80°C: about 45% of maximal activity
Haloferax volcanii
4.1.3.1
40
60
40°C: 50% of maximal activity, 60°C: about 40% of maximal activity
Haloferax volcanii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.3.9
8
9
-
Haloferax volcanii
2.3.3.9
8
-
assay at
Haloferax volcanii
4.1.3.1
7
-
-
Haloferax volcanii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.3.9
7
10
pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity
Haloferax volcanii
4.1.3.1
6.5
7.5
pH 6.5: about 70% of maximal activity, pH 7.5: about 55% of maximal activity
Haloferax volcanii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
dithiothreitol
-
Haloferax volcanii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
additional information
reversible loss of activity in isocitrate lyase from Haloferax volcanii when the enzyme is out of extract without a thiol, such as dithiothreitol
Haloferax volcanii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.3.9
0.00011
-
glyoxylate
pH 8.0, 40°C
Haloferax volcanii
2.3.3.9
0.00012
-
acetyl-CoA
pH 8.0, 40°C
Haloferax volcanii
4.1.3.1
1.16
-
D-isocitrate
pH 7.0, 40°C
Haloferax volcanii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.3.9
KCl
salt optimum: 3.0 M KCl
Haloferax volcanii
2.3.3.9
Mg2+
activity is dependent on Mg2+
Haloferax volcanii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.3.9
67000
-
3 * 67000, SDS-PAGE
Haloferax volcanii
2.3.3.9
200000
-
gel filtration
Haloferax volcanii
4.1.3.1
47000
-
3 * 47000, SDS-PAGE
Haloferax volcanii
4.1.3.1
140000
-
gel filtration
Haloferax volcanii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.3.9
acetyl-CoA + H2O + glyoxylate
Haloferax volcanii
key enzyme of the glyoxylate cycle enzyme
(S)-malate + CoA
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
Haloferax volcanii DSM 3757
key enzyme of the glyoxylate cycle enzyme
(S)-malate + CoA
-
-
?
4.1.3.1
isocitrate
Haloferax volcanii
key enzyme of the glyoxylate cycle
succinate + glyoxylate
-
-
?
4.1.3.1
isocitrate
Haloferax volcanii DSM 3757
key enzyme of the glyoxylate cycle
succinate + glyoxylate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.3.9
-
Haloferax volcanii
4.1.3.1
-
Haloferax volcanii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.3.9
culture condition:acetate-grown cell
no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is
Haloferax volcanii
-
4.1.3.1
culture condition:acetate-grown cell
no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is
Haloferax volcanii
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
727500
Haloferax volcanii
(S)-malate + CoA
-
-
-
-
2.3.3.9
acetyl-CoA + H2O + glyoxylate
key enzyme of the glyoxylate cycle enzyme
727500
Haloferax volcanii
(S)-malate + CoA
-
-
-
?
2.3.3.9
acetyl-CoA + H2O + glyoxylate
-
727500
Haloferax volcanii DSM 3757
(S)-malate + CoA
-
-
-
-
2.3.3.9
acetyl-CoA + H2O + glyoxylate
key enzyme of the glyoxylate cycle enzyme
727500
Haloferax volcanii DSM 3757
(S)-malate + CoA
-
-
-
?
4.1.3.1
D-isocitrate
-
727500
Haloferax volcanii
succinate + glyoxylate
-
-
-
?
4.1.3.1
D-isocitrate
-
727500
Haloferax volcanii DSM 3757
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
key enzyme of the glyoxylate cycle
727500
Haloferax volcanii
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
key enzyme of the glyoxylate cycle
727500
Haloferax volcanii DSM 3757
succinate + glyoxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.3.9
homotrimer
3 * 67000, SDS-PAGE
Haloferax volcanii
4.1.3.1
trimer
3 * 47000, SDS-PAGE
Haloferax volcanii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.3.9
40
-
assay at
Haloferax volcanii
2.3.3.9
50
60
-
Haloferax volcanii
4.1.3.1
40
-
assay at
Haloferax volcanii
4.1.3.1
55
-
-
Haloferax volcanii
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.3.9
25
80
25°C: about 45% of maximal activity, 80°C: about 45% of maximal activity
Haloferax volcanii
4.1.3.1
40
60
40°C: 50% of maximal activity, 60°C: about 40% of maximal activity
Haloferax volcanii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.3.9
8
9
-
Haloferax volcanii
2.3.3.9
8
-
assay at
Haloferax volcanii
4.1.3.1
7
-
-
Haloferax volcanii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.3.9
7
10
pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity
Haloferax volcanii
4.1.3.1
6.5
7.5
pH 6.5: about 70% of maximal activity, pH 7.5: about 55% of maximal activity
Haloferax volcanii
General Information
EC Number
General Information
Commentary
Organism
2.3.3.9
physiological function
key enzyme of the glyoxylate cycle enzyme
Haloferax volcanii
4.1.3.1
physiological function
key enzyme of the glyoxylate cycle
Haloferax volcanii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.3.9
physiological function
key enzyme of the glyoxylate cycle enzyme
Haloferax volcanii
4.1.3.1
physiological function
key enzyme of the glyoxylate cycle
Haloferax volcanii