EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.48 | expression in Escherichia coli | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.2.48 | at 2.2 A resolution, in the unliganded form and cocrystallized with L-serine and L-threonine. No active site catalytic residue is revealed, and a structural water molecule is assumed to act as the catalytic base in the retro-aldol cleavage reaction. The very large active site opening suggests that much larger molecules than L-threonine isomers may be easily accommodated | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.2.48 | F87A | no change in the ration of cleavage of L-threonine to L-allo-threonine | Escherichia coli |
4.1.2.48 | F87D | mutation doubles the preference of the enzyme for L-allo-threonine | Escherichia coli |
4.1.2.48 | H126F | 300% of wild-type activity,reduced preference for the erythro-substrate | Escherichia coli |
4.1.2.48 | H126N | 60% of wild-type activity | Escherichia coli |
4.1.2.48 | H83F | less than 1% of wild-type activity, reduced preference for the erythro-substrate | Escherichia coli |
4.1.2.48 | H83F/H126F | able to catalyze the cleavage of both L-threonine and L-allo-threonine at a measurable rate, neither of the histidines acts as a catalytic base in the retro-aldol cleavage mechanism | Escherichia coli |
4.1.2.48 | H83N | less than 10% of wild-type activity | Escherichia coli |
4.1.2.48 | K222A | slight decrease in kcat and slight increase in Km values for both L-threonine and L-allo-threonine | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.48 | 0.2 | - |
L-allo-threonine | mutant H126NF, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.24 | - |
L-allo-threonine | wild-type, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.4 | - |
L-threonine | mutant F87A, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.96 | - |
L-allo-threonine | mutant H126N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.1 | - |
L-threonine | mutant H83F/H126F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.3 | - |
L-allo-threonine | mutant F87A, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.3 | - |
L-threonine | mutant F87D, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.7 | - |
L-allo-threonine | mutant F87D, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.7 | - |
L-threonine | mutant H126F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.7 | - |
L-allo-threonine | mutant H83N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 3.7 | - |
L-threonine | mutant K222A, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 5 | - |
L-allo-threonine | mutant K222A, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 7 | - |
L-threonine | mutant H83F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 7 | - |
L-allo-threonine | mutant H83F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 19.4 | - |
L-threonine | wild-type, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 31 | - |
L-allo-threonine | mutant H83F/H126F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 38 | - |
L-threonine | mutant H83N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 61 | - |
L-threonine | mutant H126N, pH 7.0, 30°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.48 | Escherichia coli | - |
- |
- |
4.1.2.48 | Escherichia coli WV_060327 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.48 | L-allo-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
? | |
4.1.2.48 | L-allo-threonine | - |
Escherichia coli WV_060327 | glycine + acetaldehyde | - |
? | |
4.1.2.48 | L-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
? | |
4.1.2.48 | L-threonine | - |
Escherichia coli WV_060327 | glycine + acetaldehyde | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.48 | 0.02 | - |
L-threonine | mutant H83F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.06 | - |
L-allo-threonine | mutant H83F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.28 | - |
L-threonine | mutant H83N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.28 | - |
L-allo-threonine | mutant H83N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 1.87 | - |
L-threonine | wild-type, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 3.55 | - |
L-allo-threonine | wild-type, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 4.37 | - |
L-threonine | mutant H126N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 6 | - |
L-threonine | mutant H126F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 7.82 | - |
L-allo-threonine | mutant H126N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 9.02 | - |
L-allo-threonine | mutant H126NF, pH 7.0, 30°C | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.48 | 0.01 | - |
L-allo-threonine | mutant H83F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.01 | - |
L-threonine | mutant H83N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.07 | - |
L-threonine | mutant H126N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.1 | - |
L-threonine | wild-type, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 0.75 | - |
L-allo-threonine | mutant H83N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 3.53 | - |
L-threonine | mutant H126F, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 8.13 | - |
L-allo-threonine | mutant H126N, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 14.78 | - |
L-allo-threonine | wild-type, pH 7.0, 30°C | Escherichia coli | |
4.1.2.48 | 45.08 | - |
L-allo-threonine | mutant H126NF, pH 7.0, 30°C | Escherichia coli |