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Literature summary extracted from

  • Marchetti, M.; Bruno, S.; Campanini, B.; Peracchi, A.; Mai, N.; Mozzarelli, A.
    ATP binding to human serine racemase is cooperative and modulated by glycine (2013), FEBS J., 280, 5853-5863.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.1.18 ATP ATP binding to human serine racemase is strongly cooperative and modulated by glycine, the active-site ligand increases the serine racemase affinity for ATP by about 22fold, abolishing cooperativity. ATP increases the noncooperative glycine binding15fold Homo sapiens
5.1.1.18 glycine glycine stabilizes a protein conformation that binds ATP non-cooperatively and with high affinity, the active-site ligand increases the serine racemase affinity for ATP by about 22fold, abolishing cooperativity. ATP increases the noncooperative glycine binding 15fold Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.18 enzyme expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.18 additional information
-
 additional information Michaelis-Menten kinetics, kinetics of serine racemase alpha- and beta-elimination activities, overview Homo sapiens
5.1.1.18 40
-
 L-serine in presence of ATP, pH 8.0, 37°C Homo sapiens
5.1.1.18 48
-
 L-serine in absence of ATP, pH 8.0, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.18 L-serine Homo sapiens
-
 D-serine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.18 recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by metal affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.18 D-serine
-
 Homo sapiens L-serine
-
 r
5.1.1.18 L-serine
-
 Homo sapiens D-serine
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.18 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.18 0.048
-
 L-serine in absence of ATP, pH 8.0, 37°C Homo sapiens
5.1.1.18 0.17
-
 L-serine in presence of ATP, pH 8.0, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.18 8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.18 pyridoxal 5'-phosphate dependent on Homo sapiens

General Information

EC Number General Information Comment Organism
5.1.1.18 physiological function through cross-talk between allosteric and active sites, intracellular ATP and glycine control D-serine homeostasis, and, indirectly, NMDA receptor activity. The N-methyl D-aspartate (NMDA) receptors play a key role in excitatory neurotransmission, and control learning, memory and synaptic plasticity. Their activity is modulated by the agonist glutamate and by the co-agonists D-serine and glycine Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.1.18 0.001
-
 L-serine in absence of ATP, pH 8.0, 37°C Homo sapiens
5.1.1.18 0.0042
-
 L-serine in presence of ATP, pH 8.0, 37°C Homo sapiens