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Literature summary extracted from

  • Fukushima, T.; Mizuki, T.; Echigo, A.; Inoue, A.; Usami, R.
    Organic solvent tolerance of halophilic alpha-amylase from a Haloarchaeon, Haloarcula sp. strain S-1 (2005), Extremophiles, 9, 85-89.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.1 the enzyme is stable under NaCl concentrations between 1.8 and 4.8 M in the absence or presence of chloroform Haloarcula sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 acetone
-
 Haloarcula sp.
3.2.1.1 Cu2+ 20 mM Haloarcula sp.
3.2.1.1 Cyclohexane inhibitory effect of cyclooctane on amylase stability is stronger than that of cyclohexane Haloarcula sp.
3.2.1.1 cyclooctane inhibitory effect of cyclooctane on amylase stability is stronger than that of cyclohexane Haloarcula sp.
3.2.1.1 EDTA 5–15 mM, with or without chloroform Haloarcula sp.
3.2.1.1 ethanol
-
 Haloarcula sp.
3.2.1.1 Fe2+ 20 mM Haloarcula sp.
3.2.1.1 Fe3+ 20 mM Haloarcula sp.
3.2.1.1 additional information not inhibited by aromatic hydrocarbon (benzene, etc.) Haloarcula sp.
3.2.1.1 Ni2+ 20 mM Haloarcula sp.
3.2.1.1 Rb+ 20 mM Haloarcula sp.
3.2.1.1 Zn2+ 20 mM Haloarcula sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Haloarcula sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 NaCl optimal NaCl concentration in the absence of chloroform is 4.3 M. No activity is detected below 1.7 M NaCl. In the presence of chloroform, optimal NaCl concentration is 4.3 M, and activity is not detected below 0.9 M Haloarcula sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 68000
-
 gel filtration Haloarcula sp.
3.2.1.1 70000
-
 1 * 70000, SDS-PAGE Haloarcula sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.1 benzene stable in presence of Haloarcula sp.
3.2.1.1 chloroform stable in presence of Haloarcula sp.
3.2.1.1 n-decane stable in presence of Haloarcula sp.
3.2.1.1 n-nonane stable in presence of Haloarcula sp.
3.2.1.1 octane stable in presence of Haloarcula sp.
3.2.1.1 styrene stable in presence of Haloarcula sp.
3.2.1.1 toluene stable in presence of Haloarcula sp.
3.2.1.1 xylene stable in presence of Haloarcula sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Haloarcula sp.
-
-
-
3.2.1.1 Haloarcula sp. S-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Haloarcula sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
 0.07 mg of starch/min*mg Haloarcula sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O
-
 Haloarcula sp. maltose + maltotriose + D-glucose major reaction products of the amylase are maltose and maltotriose, and the minor product is glucose ?
3.2.1.1 starch + H2O
-
 Haloarcula sp. S-1 maltose + maltotriose + D-glucose major reaction products of the amylase are maltose and maltotriose, and the minor product is glucose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 70000, SDS-PAGE Haloarcula sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 50
-
 in buffer containing 4.3 M NaCl, pH 7.0 Haloarcula sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 37
-
 pH 7.0, 50 mM Tris-HCl buffer containing 4.3 M NaCl, 30 min, no loss of activity Haloarcula sp.
3.2.1.1 70
-
 pH 7.0, 50 mM Tris-HCl buffer containing 4.3 M NaCl, 30 min, 50% loss of activity Haloarcula sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 7.5
-
-
 Haloarcula sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 6 8 25°C, 30 min, stable Haloarcula sp.