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Literature summary extracted from

  • Siroosi, M.; Amoozegar, M.A.; Khajeh, K.; Fazeli, M.; Rezaei, M.H.
    Purification and characterization of a novel extracellular halophilic and organic solvent-tolerant amylopullulanase from the haloarchaeon, Halorubrum sp. strain Ha25 (2014), Extremophiles, 18, 25-33.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 additional information
-
 soluble starch Km-value: 1.8 mg/ml Halorubrum sp. Ha25
3.2.1.41 additional information
-
 pullulan KM-value: 4 mg/ml Halorubrum sp. Ha25

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
 Halorubrum sp. Ha25
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 NaCl the enzyme is active over a wide range of concentrations (0–4.5 M) of NaCl Halorubrum sp. Ha25

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 14000
-
 x * 14000, SDS-PAGE Halorubrum sp. Ha25

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.41 Acetone 20%, half-life of pullulytic activity: 155 h, half-life of amylolytic activity: 255 h Halorubrum sp. Ha25
3.2.1.41 chloroform 20%, half-life of pullulytic activity: 150 h, half-life of amylolytic activity: 230 h Halorubrum sp. Ha25
3.2.1.41 dimethyl sulfoxide 20%, half-life of pullulytic activity: 46 h, half-life of amylolytic activity: 41 h Halorubrum sp. Ha25
3.2.1.41 Ethanol 20%, half-life of pullulytic activity: 57 h, half-life of amylolytic activity: 99 h Halorubrum sp. Ha25
3.2.1.41 additional information the enzyme is more stable in the presence of non-polar organic solvents than polar solvents (20%) Halorubrum sp. Ha25
3.2.1.41 N,N-dimethylformamide 20%, half-life of pullulytic activity: 65 h, half-life of amylolytic activity: 101 h Halorubrum sp. Ha25
3.2.1.41 n-Butanol 20%, half-life of pullulytic activity: 125 h, half-life of amylolytic activity: 215 h Halorubrum sp. Ha25
3.2.1.41 propanol 20%, half-life of pullulytic activity: 33 h, half-life of amylolytic activity: 53 h Halorubrum sp. Ha25

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Halorubrum sp. Ha25
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Halorubrum sp. Ha25

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 pullulan + H2O
-
 Halorubrum sp. Ha25 ?
-
 ?
3.2.1.41 soluble starch + H2O
-
 Halorubrum sp. Ha25 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 14000, SDS-PAGE Halorubrum sp. Ha25

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 amylopullulanase
-
 Halorubrum sp. Ha25

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 50
-
-
 Halorubrum sp. Ha25

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 40 65 40°C: about 45% of maximal activity, 65°C: about 55% of maximal activity Halorubrum sp. Ha25

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 50
-
 half-life: 155 min Halorubrum sp. Ha25
3.2.1.41 60
-
 half-life: 43 min Halorubrum sp. Ha25
3.2.1.41 70
-
 half-life: 21 min Halorubrum sp. Ha25

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 7
-
 amylolytic activity Halorubrum sp. Ha25
3.2.1.41 7.5
-
 pullulytic activity Halorubrum sp. Ha25

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 5.5 8.5 pH 5.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity Halorubrum sp. Ha25