EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.33 | the TK2141 gene encoding PoK, with a His6-Tag on its N-terminus, is overexpressed in Thermococcus kodakarensis | Thermococcus kodakarensis |
6.3.2.26 | overexpressed in Escherichoa coli | Thermococcus kodakarensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.33 | 4-phosphopantoate | substrate inhibition | Thermococcus kodakarensis | |
2.7.1.33 | ATP | substrate inhibition | Thermococcus kodakarensis | |
2.7.1.33 | additional information | feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed | Thermococcus kodakarensis | |
6.3.2.26 | 4-phosphopantoate | substrate inhibition | Thermococcus kodakarensis | |
6.3.2.26 | ATP | substrate inhibition | Thermococcus kodakarensis | |
6.3.2.26 | additional information | feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed | Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.2.26 | 0.34 | - |
beta-Alanine | pH 6.5, 85°C | Thermococcus kodakarensis | |
6.3.2.26 | 1.16 | - |
(R)-4-phosphopantoate | pH 6.5, 85°C | Thermococcus kodakarensis | |
6.3.2.26 | 2.44 | - |
ATP | pH 6.5, 85°C | Thermococcus kodakarensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + (R)-4-phosphopantoate + beta-alanine | Thermococcus kodakarensis | the enzyme is involved in coenzyme A biosynthesis in the archaea | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
6.3.2.26 | ATP + (R)-4-phosphopantoate + beta-alanine | Thermococcus kodakarensis | the enzyme is involved in coenzyme A biosynthesis in the archaea | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.33 | Thermococcus kodakarensis | Q5JHF1 | - |
- |
6.3.2.26 | Thermococcus kodakarensis | Q5JIZ8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.33 | - |
Thermococcus kodakarensis |
6.3.2.26 | - |
Thermococcus kodakarensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.2.26 | 6.38 | - |
beta-alanine, pH 6.5, 85°C | Thermococcus kodakarensis |
6.3.2.26 | 14.7 | - |
ATP, pH 6.5, 85°C | Thermococcus kodakarensis |
6.3.2.26 | 15.6 | - |
(R)-4-phosphopantoate, pH 6.5, 85°C | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + (R)-4-phosphopantoate + beta-alanine | the enzyme is involved in coenzyme A biosynthesis in the archaea | Thermococcus kodakarensis | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
6.3.2.26 | ATP + (R)-4-phosphopantoate + beta-alanine | the enzyme is involved in coenzyme A biosynthesis in the archaea | Thermococcus kodakarensis | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
6.3.2.26 | ATP + (R)-4-phosphopantoate + beta-alanine | strict preference for ATP. Among several amine substrates, activity is detected with beta-alanine, but not with gamma-aminobutyrate, glycine nor aspartate | Thermococcus kodakarensis | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.33 | 4-phosphopantoate | non-competitive | Thermococcus kodakarensis |
2.7.1.33 | PoK | - |
Thermococcus kodakarensis |
2.7.1.33 | TK2141 | - |
Thermococcus kodakarensis |
6.3.2.26 | phosphopantothenate synthetase | - |
Thermococcus kodakarensis |
6.3.2.26 | Pps | - |
Thermococcus kodakarensis |
6.3.2.26 | TK1686 | - |
Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.33 | 75 | - |
assay at | Thermococcus kodakarensis |
6.3.2.26 | 85 | - |
assay at | Thermococcus kodakarensis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.2.26 | 65 | 95 | activity continues to increase with temperature elevation from 65°C up to 95°C | Thermococcus kodakarensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.2.26 | 70 | - |
6 h, no decrease in activity | Thermococcus kodakarensis |
6.3.2.26 | 80 | - |
half-life: 13.6 h | Thermococcus kodakarensis |
6.3.2.26 | 90 | - |
half-life: 8.2 h | Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.33 | 8 | - |
assay at | Thermococcus kodakarensis |
6.3.2.26 | 6.5 | - |
- |
Thermococcus kodakarensis |