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Literature summary extracted from
- Effects of the N-terminal and C-terminal domains of Meiothermus ruber CBS-01 trehalose synthase on thermostability and activity (2012), Extremophiles, 16, 377-385.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | gene treS, sequence comparison, expression of His6-tagged wild-type enzyme and chimeric mutant in Escherichia coli strain Rosetta-gami (DE3), subcloning in Escherichia coli strain DH5alpha | Thermus thermophilus |
| 5.4.99.16 | gene treS, sequence comparison, expression of His6-tagged wild-type enzyme and the chimeric mutant in Escherichia coli strain Rosetta-gami (DE3), subcloning in Escherichia coli strain DH5alpha | Meiothermus ruber |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | additional information | construction of a chimeric enzyme mutant consisting of the C-terminus from Meiothermus ruber trehalose synthase and the N-terminus from Thermus thermophilus trehalose synthase, TSMrTt , and a second with N-terminus from Meiothermus ruber trehalose synthase and C-terminus from Thermus thermophilus trehalose synthase, TSTtMr | Meiothermus ruber |
| 5.4.99.16 | additional information | construction of a chimeric enzyme mutant consisting of the C-terminus from Meiothermus ruber trehalose synthase and the N-terminus from Thermus thermophilus trehalose synthase, TSMrTt , and a second with N-terminus from Meiothermus ruber trehalose synthase and C-terminus from Thermus thermophilus trehalose synthase, TSTtMr | Thermus thermophilus |
| 5.4.99.16 | R392A | site-directed mutagenesis, the mutant shows a sharp decrease in activity compared to the wild-type enzyme | Meiothermus ruber |
| 5.4.99.16 | R392F | site-directed mutagenesis, the mutation leads to a complete loss in activity | Meiothermus ruber |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 57.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber |  |
| 5.4.99.16 | 57.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
| 5.4.99.16 | 69.6 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
| 5.4.99.16 | 69.6 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
| 5.4.99.16 | 79.2 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
| 5.4.99.16 | 79.2 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
| 5.4.99.16 | 82.2 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
| 5.4.99.16 | 97.4 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
| 5.4.99.16 | 98.9 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 126 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 154 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
| 5.4.99.16 | 154 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | Meiothermus ruber | - |
alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | Thermus thermophilus | - |
alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | Meiothermus ruber CBS-01 | - |
alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
alpha,alpha-trehalose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.16 | Meiothermus ruber | B1PK99 | gene treS | - |
| 5.4.99.16 | Meiothermus ruber CBS-01 | B1PK99 | gene treS | - |
| 5.4.99.16 | Thermus thermophilus | Q5SL15 | gene treS | - |
| 5.4.99.16 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SL15 | gene treS | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | recombinant His6-tagged wild-type and chimeric mutant enzymes from Escherichia coli strain Rosetta-gami (DE3) by nickel affinity chromatography | Meiothermus ruber |
| 5.4.99.16 | recombinant His6-tagged wild-type and chimeric mutant enzymes from Escherichia coli strain Rosetta-gami (DE3) by nickel affinity chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | alpha,alpha-trehalose | - |
Meiothermus ruber | maltose | - |
r | |
| 5.4.99.16 | alpha,alpha-trehalose | - |
Thermus thermophilus | maltose | - |
? | |
| 5.4.99.16 | alpha,alpha-trehalose | - |
Meiothermus ruber CBS-01 | maltose | - |
r | |
| 5.4.99.16 | alpha,alpha-trehalose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | maltose | - |
? | |
| 5.4.99.16 | maltose | - |
Meiothermus ruber | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | - |
Thermus thermophilus | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | - |
Meiothermus ruber CBS-01 | alpha,alpha-trehalose | - |
r | |
| 5.4.99.16 | maltose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | alpha,alpha-trehalose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | More | the isolated N-terminal domain from Meiothermus ruber is not active. The secondary structure of the isolated N-terminal domain undergoes a greater change than that of the isolated C-terminus, three-dimensional structure analysis and modeling, overview | Thermus thermophilus |
| 5.4.99.16 | More | the isolated N-terminal domain from Meiothermus ruber is not active. The secondary structure of the isolated N-terminal domain undergoes a greater change than that of the isolated C-terminus, three-dimensional structure enzyme structure analysis and molecular modeling, overview | Meiothermus ruber |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | Trehalose synthase | - |
Meiothermus ruber |
| 5.4.99.16 | Trehalose synthase | - |
Thermus thermophilus |
| 5.4.99.16 | TreS | - |
Meiothermus ruber |
| 5.4.99.16 | TreS | - |
Thermus thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 50 | - |
recombinant wild-type enzyme and mutant TSMrTt | Meiothermus ruber |
| 5.4.99.16 | 60 | - |
recombinant wild-type enzyme and mutant TSTtMr | Thermus thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 70 | - |
half-lives of purified wild-type enzyme: 48.5 min, purified mutant TSMrTt : 47.6 min, and purified mutant TSTtMr: over 480 min, mutant R392A: 42.2 min | Meiothermus ruber |
| 5.4.99.16 | 70 | - |
half-lives of purified wild-type enzyme: over 480 min, purified mutant TSMrTt : 47.6 min, and purified mutant TSTtMr: over 480 min | Thermus thermophilus |
| 5.4.99.16 | 75 | - |
half-lives of purified wild-type enzyme: 15.8 min, purified mutant TSMrTt : 17.6 min, and purified mutant TSTtMr: over 480 min, mutant R392A: 12.5 min | Meiothermus ruber |
| 5.4.99.16 | 75 | - |
half-lives of purified wild-type enzyme: 475 min, purified mutant TSMrTt : 17.6 min, and purified mutant TSTtMr: over 480 min | Thermus thermophilus |
| 5.4.99.16 | 80 | - |
half-lives of purified wild-type enzyme: 233 min, purified mutant TSMrTt : below 5 min, and purified mutant TSTtMr: 27.6 min | Thermus thermophilus |
| 5.4.99.16 | 80 | - |
half-lives of purified wild-type enzyme: below 5 min, purified mutant TSMrTt : below 5 min, and purified mutant TSTtMr: 27.6 min, mutant R392A: below 5 min | Meiothermus ruber |
| 5.4.99.16 | 100 | - |
purified enzyme, 10 min, inactivation | Meiothermus ruber |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 61 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
| 5.4.99.16 | 68.9 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 110 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 115 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
| 5.4.99.16 | 115 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
| 5.4.99.16 | 147 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 152 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
| 5.4.99.16 | 152 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
| 5.4.99.16 | 202 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
| 5.4.99.16 | 227 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 6.5 | - |
assay at | Meiothermus ruber |
| 5.4.99.16 | 6.5 | - |
assay at | Thermus thermophilus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | additional information | enzyme structure analysis and molecular modeling, overview | Meiothermus ruber |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 0.697 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 0.871 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
| 5.4.99.16 | 0.871 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
| 5.4.99.16 | 1.16 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
| 5.4.99.16 | 1.32 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
| 5.4.99.16 | 1.32 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
| 5.4.99.16 | 1.33 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
| 5.4.99.16 | 1.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
| 5.4.99.16 | 1.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
| 5.4.99.16 | 2.51 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
| 5.4.99.16 | 2.7 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
| 5.4.99.16 | 2.7 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
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