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Literature summary extracted from

  • Hansen, T.; Arnfors, L.; Ladenstein, R.; Schönheit, P.
    The phosphofructokinase-B (MJ0406) from Methanocaldococcus jannaschii represents a nucleoside kinase with a broad substrate specificity (2006), Extremophiles, 11, 105-114.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.B20 expressed in Escherichia coli Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.B20 0.017
-
 cytidine pH 7.0, 50°C, cosubstrate: ATP Methanocaldococcus jannaschii
2.7.1.B20 0.021
-
 Inosine pH 7.0, 50°C, cosubstrate: ATP Methanocaldococcus jannaschii
2.7.1.B20 0.062
-
 guanosine pH 7.0, 70°C, cosubstrate: ATP Methanocaldococcus jannaschii
2.7.1.B20 0.078
-
 guanosine pH 7.0, 50°C, cosubstrate: ATP Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.B20 Co2+ enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) Methanocaldococcus jannaschii
2.7.1.B20 Mg2+ enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) Methanocaldococcus jannaschii
2.7.1.B20 Mn2+ enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) Methanocaldococcus jannaschii
2.7.1.B20 Ni2+ enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.B20 34000
-
 2 * 34000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.B20 68000
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.B20 additional information Methanocaldococcus jannaschii the enzyme plays a role in nucleotide metabolism ?
-
 ?
2.7.1.B20 additional information Methanocaldococcus jannaschii DSM 2661 the enzyme plays a role in nucleotide metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.B20 Methanocaldococcus jannaschii Q57849
-
-
2.7.1.B20 Methanocaldococcus jannaschii DSM 2661 Q57849
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.B20
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.B20 ATP + cytidine at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP Methanocaldococcus jannaschii ADP + CMP
-
 ?
2.7.1.B20 ATP + cytidine at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP Methanocaldococcus jannaschii DSM 2661 ADP + CMP
-
 ?
2.7.1.B20 ATP + guanosine at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP Methanocaldococcus jannaschii ADP + GMP
-
 ?
2.7.1.B20 ATP + guanosine at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP Methanocaldococcus jannaschii DSM 2661 ADP + GMP
-
 ?
2.7.1.B20 ATP + inosine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii ADP + IMP
-
 ?
2.7.1.B20 ATP + inosine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii DSM 2661 ADP + IMP
-
 ?
2.7.1.B20 GTP + cytidine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii GDP + CMP
-
 ?
2.7.1.B20 GTP + guanosine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii GDP + GMP
-
 ?
2.7.1.B20 GTP + inosine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii GDP + IMP
-
 ?
2.7.1.B20 ITP + cytidine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii IDP + CMP
-
 ?
2.7.1.B20 ITP + guanosine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii IDP + GMP
-
 ?
2.7.1.B20 ITP + inosine relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) Methanocaldococcus jannaschii IDP + IMP
-
 ?
2.7.1.B20 additional information the enzyme plays a role in nucleotide metabolism Methanocaldococcus jannaschii ?
-
 ?
2.7.1.B20 additional information adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate Methanocaldococcus jannaschii ?
-
 ?
2.7.1.B20 additional information the enzyme plays a role in nucleotide metabolism Methanocaldococcus jannaschii DSM 2661 ?
-
 ?
2.7.1.B20 additional information adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate Methanocaldococcus jannaschii DSM 2661 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.B20 homodimer 2 * 34000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.B20 MJ0406 locus name Methanocaldococcus jannaschii
2.7.1.B20 NTP:nucleoside kinase
-
 Methanocaldococcus jannaschii
2.7.1.B20 PFK-B sugar kinase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.B20 85
-
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.B20 90
-
 melting temperature Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.B20 7
-
-
 Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.B20 6.1 8.2 50% of maximal activity at pH 6.1 and at pH 8.2 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.7.1.B20 physiological function the enzyme plays a role in nucleotide metabolism Methanocaldococcus jannaschii