Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Skedelj, V.; Perdih, A.; Brvar, M.; Kroflic, A.; Dubbee, V.; Savage, V.; O'Neill, A.J.; Solmajer, T.; Bester-Rogac, M.; Blanot, D.; Hugonnet, J.E.; Magnet, S.; Arthur, M.; Mainardi, J.L.; Stojan, J.; Zega, A.
    Discovery of the first inhibitors of bacterial enzyme D-aspartate ligase from Enterococcus faecium (Aslfm) (2013), Eur. J. Med. Chem., 67, 208-220.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.1.12 drug development D-aspartate ligase of Enterococcus faecium is an attractive target for the development of narrow-spectrum antibacterial agents that are active against multidrug-resistant Enterococcus faecium Enterococcus faecium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.12 His-tagged enzyme expression in Escherichia coli strain BL21 (lDE3)/pREP4 Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.12 1-(4-methylphenyl)-5-[5-(3-methylphenyl)-1H-pyrazol-4-yl]-1H-tetrazole
-
 Enterococcus faecium
6.3.1.12 1H-imidazol-4-yl(diphenyl)methanol
-
 Enterococcus faecium
6.3.1.12 2-amino-N,N-bis(pyridin-2-ylmethyl)oxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N,N-dibenzyl-4-methylthiazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N,N-dibenzyloxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N,N-dibenzylthiazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N,N-dibutyloxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N-(1H-benzo[d]imidazol-2-yl)oxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N-benzyl-N-butyloxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-amino-N-ethyl-N-(pyridin-4-ylmethyl)oxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 2-[2-(4-amino-1,2,5-oxadiazol-3-yl)-1H-benzimidazol-1-yl]-N-methyl-N-phenylacetamide
-
 Enterococcus faecium
6.3.1.12 2-[2-(4-amino-1,2,5-oxadiazol-3-yl)-1H-benzimidazol-1-yl]-N-phenylacetamide
-
 Enterococcus faecium
6.3.1.12 2-[2-[(methylsulfonyl)amino]-1,3-thiazol-4-yl]-N,N-bis(thiophen-2-ylmethyl)acetamide
-
 Enterococcus faecium
6.3.1.12 4-phenyl-5-(1-phenyl-1H-tetrazol-5-yl)pyrimidin-2-amine
-
 Enterococcus faecium
6.3.1.12 5-(diphenylphosphoryl)-1,3,4-thiadiazol-2-amine
-
 Enterococcus faecium
6.3.1.12 6-[(2,4-dichlorobenzyl)sulfanyl]-9H-purin-2-amine
-
 Enterococcus faecium
6.3.1.12 6-[(2,6-dichlorobenzyl)sulfanyl]-9H-purin-2-amine
-
 Enterococcus faecium
6.3.1.12 additional information screening of a series of amino-oxazoles derived from bacterial biotin carboxylase inhibitors, the compounds show low micromolar activity against D-aspartate ligase. Screening of enzyme inhibitors with 7-amino-9H-purine and 7-amino-1H-pyrazolo[3,4-d]pyrimidine scaffolds, the compounds are competitive with respect to the ATP molecule Enterococcus faecium
6.3.1.12 N,N-dibenzyl-9H-purin-6-amine
-
 Enterococcus faecium
6.3.1.12 N-((1H-indol-5-yl)methyl)-2-amino-N-(3,5-dibromobenzyl)oxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 N-((1H-indol-5-yl)methyl)-2-aminooxazole-5-carboxamide
-
 Enterococcus faecium
6.3.1.12 N-(furan-2-ylmethyl)-5-hydroxy-N-(thiophen-2-ylmethyl)pyrazine-2-carboxamide
-
 Enterococcus faecium
6.3.1.12 N-(furan-2-ylmethyl)-6-hydroxy-N-(thiophen-2-ylmethyl)pyridine-3-carboxamide
-
 Enterococcus faecium
6.3.1.12 N-benzyl-N-(2,6-dichlorobenzyl)-9H-purin-6-amine
-
 Enterococcus faecium
6.3.1.12 N-benzyl-N-(4-chlorobenzyl)-9H-purin-6-amine
-
 Enterococcus faecium
6.3.1.12 N4-(2,3-dichlorobenzyl)-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-(2,4-dichlorobenzyl)-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-(2,4-difluorobenzyl)-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-(2,6-difluoro-3-methylbenzyl)-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-(2,6-difluorobenzyl)-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-(2-chloro-6-fluorobenzyl)-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-[(2-chloropyridin-3-yl)methyl]-N4-methyl-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N4-[(5-chlorothiophen-2-yl)methyl]-1H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
-
 Enterococcus faecium
6.3.1.12 N6-(1,3-benzodioxol-5-ylmethyl)-9H-purine-2,6-diamine
-
 Enterococcus faecium
6.3.1.12 N6-(2,4-dichlorobenzyl)-9H-purine-2,6-diamine
-
 Enterococcus faecium
6.3.1.12 N6-(2,4-dimethoxybenzyl)-9H-purine-2,6-diamine
-
 Enterococcus faecium
6.3.1.12 N6-(3,4-dichlorobenzyl)-9H-purine-2,6-diamine
-
 Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.12 ATP + D-aspartate + [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n Enterococcus faecium
-
 [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate
-
 ?
6.3.1.12 ATP + D-aspartate + [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n Enterococcus faecium D359
-
 [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.12 Enterococcus faecium
-
-
-
6.3.1.12 Enterococcus faecium D359
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.12 recombinant His-tagged enzyme from Escherichia coli strain BL21 (lDE3)/pREP4 by nickel affinity chromatography, gel filtration, and ultrafiltration Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.12 ATP + D-aspartate + [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n
-
 Enterococcus faecium [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate
-
 ?
6.3.1.12 ATP + D-aspartate + [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n
-
 Enterococcus faecium D359 [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.12 Aslfm
-
 Enterococcus faecium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.12 37
-
 assay at Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.12 8
-
 assay at Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.12 ATP
-
 Enterococcus faecium

General Information

EC Number General Information Comment Organism
6.3.1.12 evolution the enzyme belongs to the ATP-grasp superfamily Enterococcus faecium