EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.26 | cloned and expressed in Escherichia coli | Thermococcus litoralis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.26 | 4-chloromercuribenzoate | 1 mM, 95% inhibition | Thermococcus litoralis | |
1.1.1.26 | CuCl2 | 1 mM, 66% inhibition | Thermococcus litoralis | |
1.1.1.26 | dithiothreitol | 1 mM, 50% inhibition | Thermococcus litoralis | |
1.1.1.26 | HgCl2 | 1 mM, complete inhibition | Thermococcus litoralis | |
1.1.1.26 | additional information | a significant effect on the activity is not observed with 1 mM EDTA, 1 mM iodoacetate and 1 mM 2-mercaptoethanol | Thermococcus litoralis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.26 | 0.067 | - |
NADH | pH 6.5, 50°C | Thermococcus litoralis | |
1.1.1.26 | 0.73 | - |
glyoxylate | pH 6.5, 50°C | Thermococcus litoralis | |
1.1.1.26 | 1.3 | - |
Hydroxypyruvate | pH 6.5, 50°C | Thermococcus litoralis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.26 | 36807 | - |
2 * 36807, calculated from sequence | Thermococcus litoralis |
1.1.1.26 | 37000 | - |
2 * 37000, SDS-PAGE | Thermococcus litoralis |
1.1.1.26 | 76000 | - |
non-denaturimg gradient PAGE | Thermococcus litoralis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.26 | glycolate + NAD+ | Thermococcus litoralis | - |
glyoxylate + NADH + H+ | - |
? | |
1.1.1.26 | glycolate + NAD+ | Thermococcus litoralis DSM 5473 | - |
glyoxylate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.26 | Thermococcus litoralis | Q9C4M5 | - |
- |
1.1.1.26 | Thermococcus litoralis DSM 5473 | Q9C4M5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.26 | - |
Thermococcus litoralis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.26 | 44.5 | - |
pH 6.5, 50°C | Thermococcus litoralis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.26 | glycolate + NAD+ | - |
Thermococcus litoralis | glyoxylate + NADH + H+ | - |
? | |
1.1.1.26 | glycolate + NAD+ | - |
Thermococcus litoralis DSM 5473 | glyoxylate + NADH + H+ | - |
? | |
1.1.1.26 | glyoxylate + NADH + H+ | NADPH exhibits 7.5% of the activity compared to NADH | Thermococcus litoralis | glycolate + NAD+ | - |
? | |
1.1.1.26 | glyoxylate + NADH + H+ | NADPH exhibits 7.5% of the activity compared to NADH | Thermococcus litoralis DSM 5473 | glycolate + NAD+ | - |
? | |
1.1.1.26 | hydroxypyruvate + NADH + H+ | the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor | Thermococcus litoralis | D-glycerate + NAD+ | - |
? | |
1.1.1.26 | hydroxypyruvate + NADH + H+ | the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor | Thermococcus litoralis DSM 5473 | D-glycerate + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.26 | homodimer | 2 * 37000, SDS-PAGE | Thermococcus litoralis |
1.1.1.26 | homodimer | 2 * 36807, calculated from sequence | Thermococcus litoralis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.26 | NADH-dependent glyoxylate reductase | - |
Thermococcus litoralis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.26 | 50 | - |
assay at | Thermococcus litoralis |
1.1.1.26 | 90 | - |
- |
Thermococcus litoralis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.26 | 90 | - |
10 min, stable up to | Thermococcus litoralis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.26 | 6.5 | - |
- |
Thermococcus litoralis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.26 | NADH | NADPH exhibits rather low activity as an electron donor compared with NADH | Thermococcus litoralis |