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Literature summary extracted from

  • Jeon, E.J.; Jung, J.H.; Seo, D.H.; Jung, D.H.; Holden, J.F.; Park, C.S.
    Bioinformatic and biochemical analysis of a novel maltose-forming alpha-amylase of the GH57 family in the hyperthermophilic archaeon Thermococcus sp. CL1 (2014), Enzyme Microb. Technol., 60, 9-15.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.2 expressed the gene in Escherichia coli Thermococcus sp.
3.2.1.B36 expressed the gene in Escherichia coli Thermococcus sp.
3.2.1.133 expressed the gene in Escherichia coli Thermococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.2 4.25
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.2 16.2
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.2 18.2
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 0.7
-
 6-O-maltotetraosyl-beta-cyclodextrin pH 6.0, 98°C Thermococcus sp.
3.2.1.B36 4.25
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 16.2
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 18.2
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 4.25
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 16.2
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 18.2
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.2 intracellular
-
 Thermococcus sp. 5622
-
3.2.1.B36 intracellular
-
 Thermococcus sp. 5622
-
3.2.1.133 intracellular
-
 Thermococcus sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 67120
-
-
 Thermococcus sp.
3.2.1.2 70000
-
 1 * 70000, SDS-PAGE Thermococcus sp.
3.2.1.B36 67120
-
-
 Thermococcus sp.
3.2.1.B36 70000
-
 1 * 70000, SDS-PAGE Thermococcus sp.
3.2.1.133 67120
-
-
 Thermococcus sp.
3.2.1.133 70000
-
 1 * 70000, SDS-PAGE Thermococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Thermococcus sp. I3ZTN9
-
-
3.2.1.2 Thermococcus sp. CL1 I3ZTN9
-
-
3.2.1.B36 Thermococcus sp. I3ZTN9
-
-
3.2.1.B36 Thermococcus sp. CL1 I3ZTN9
-
-
3.2.1.133 Thermococcus sp. I3ZTN9
-
-
3.2.1.133 Thermococcus sp. CL1 I3ZTN9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 amylopectin + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.2 amylopectin + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. CL1 maltose + ?
-
 ?
3.2.1.2 glycogen + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.2 glycogen + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. CL1 maltose + ?
-
 ?
3.2.1.2 maltopentaose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. 2 maltose + D-glucose
-
 ?
3.2.1.2 maltopentaose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. CL1 2 maltose + D-glucose
-
 ?
3.2.1.2 maltotetraose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. 2 maltose
-
 ?
3.2.1.2 maltotetraose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. CL1 2 maltose
-
 ?
3.2.1.2 maltotriose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. maltose + D-glucose
-
 ?
3.2.1.2 maltotriose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. CL1 maltose + D-glucose
-
 ?
3.2.1.2 soluble starch + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.B36 6-O-maltosyl-beta-cyclodextrin + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. maltose + beta-cyclodextrin
-
 ?
3.2.1.B36 6-O-maltotetraosyl-beta-cyclodextrin + H2O
-
 Thermococcus sp. 2 maltose + beta-cyclodextrin
-
 ?
3.2.1.B36 amylopectin + H2O the enzyme only released maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.B36 glycogen + H2O the enzyme only released maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.B36 maltopentaose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. 2 maltose + D-glucose
-
 ?
3.2.1.B36 maltotetraose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. 2 maltose
-
 ?
3.2.1.B36 maltotriose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. maltose + D-glucose
-
 ?
3.2.1.B36 soluble starch + H2O the enzyme only released maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.133 amylopectin + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.133 glycogen + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?
3.2.1.133 maltopentaose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. 2 maltose + D-glucose
-
 ?
3.2.1.133 maltotetraose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. 2 maltose
-
 ?
3.2.1.133 maltotriose + H2O the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible Thermococcus sp. maltose + D-glucose
-
 ?
3.2.1.133 soluble starch + H2O the enzyme only releases maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan Thermococcus sp. maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.2 monomer 1 * 70000, SDS-PAGE Thermococcus sp.
3.2.1.B36 monomer 1 * 70000, SDS-PAGE Thermococcus sp.
3.2.1.133 monomer 1 * 70000, SDS-PAGE Thermococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.2 TCMA
-
 Thermococcus sp.
3.2.1.B36 TCMA
-
 Thermococcus sp.
3.2.1.133 TCMA
-
 Thermococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 85
-
 alpha-1,4-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.2 98
-
 alpha-1,6-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.B36 85
-
 alpha-1,4-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.B36 98
-
 alpha-1,6-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.133 85
-
 alpha-1,4-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.133 98
-
 alpha-1,6-glycosidic linkage hydrolysis Thermococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.2 60 95 60°C; about 50% of maximal activity, 60°C: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose Thermococcus sp.
3.2.1.2 80 98 activity at 80°C is about 50% compared to the activity at 98°C, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin Thermococcus sp.
3.2.1.B36 60 95 60°C; about 50% of maximal activity, 60°C: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose Thermococcus sp.
3.2.1.B36 80 98 activity at 80°C is about 50% compared to the activity at 98°C, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin Thermococcus sp.
3.2.1.133 60 95 60°C; about 50% of maximal activity, 60°C: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose Thermococcus sp.
3.2.1.133 80 98 activity at 80°C is about 50% compared to the activity at 98°C, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin Thermococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.2 67
-
 melting temperature at pH 4.0 Thermococcus sp.
3.2.1.2 85
-
 the half-life is 69 min, 225 min, and 255 min at pH 5.0, pH 6.0, and pH 7.0 Thermococcus sp.
3.2.1.2 91
-
 melting temperature at pH 5.0 Thermococcus sp.
3.2.1.2 105
-
 melting temperature at pH 6.0 Thermococcus sp.
3.2.1.B36 67
-
 melting temperature at pH 4.0 Thermococcus sp.
3.2.1.B36 85
-
 the half-life is 69 min, 225 min, and 255 min at pH 5.0, pH 6.0, and pH 7.0 Thermococcus sp.
3.2.1.B36 91
-
 melting temperature at pH 5.0 Thermococcus sp.
3.2.1.B36 105
-
 melting temperature at pH 6.0 Thermococcus sp.
3.2.1.133 67
-
 melting temperature at pH 4.0 Thermococcus sp.
3.2.1.133 85
-
 the half-life is 69 min, 225 min, and 255 min at pH 5.0, pH 6.0, and pH 7.0 Thermococcus sp.
3.2.1.133 91
-
 melting temperature at pH 5.0 Thermococcus sp.
3.2.1.133 105
-
 melting temperature at pH 6.0 Thermococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.2 3.85
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.2 3.95
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.2 11.45
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 3.85
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 3.95
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 11.45
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 30.1
-
 6-O-maltotetraosyl-beta-cyclodextrin pH 6.0, 98°C Thermococcus sp.
3.2.1.133 3.85
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 3.95
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 11.45
-
 maltotriose pH 5.0, 85°C Thermococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 5
-
 alpha-1,4-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.2 6
-
 alpha-1,6-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.B36 5
-
 alpha-1,4-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.B36 6
-
 alpha-1,6-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.133 5
-
 alpha-1,4-glycosidic linkage hydrolysis Thermococcus sp.
3.2.1.133 6
-
 alpha-1,6-glycosidic linkage hydrolysis Thermococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.2 4 6 pH 4.0: about 85% of maximal activity, pH 6.0: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose Thermococcus sp.
3.2.1.2 4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin Thermococcus sp.
3.2.1.B36 4 6 pH 4.0: about 85% of maximal activity, pH 6.0: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose Thermococcus sp.
3.2.1.B36 4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin Thermococcus sp.
3.2.1.133 4 6 pH 4.0: about 85% of maximal activity, pH 6.0: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose Thermococcus sp.
3.2.1.133 4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin Thermococcus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.2 0.22
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.2 0.24
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.2 2.7
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 0.22
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 0.24
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 2.7
-
 maltotriose pH 5.0, 85°C Thermococcus sp.
3.2.1.B36 42.8
-
 6-O-maltotetraosyl-beta-cyclodextrin pH 6.0, 98°C Thermococcus sp.
3.2.1.133 0.22
-
 maltotetraose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 0.24
-
 maltopentaose pH 5.0, 85°C Thermococcus sp.
3.2.1.133 2.7
-
 maltotriose pH 5.0, 85°C Thermococcus sp.