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Literature summary extracted from

  • Goulter, K.C.; Hashimi, S.M.; Birch, R.G.
    Microbial sucrose isomerases: producing organisms, genes and enzymes (2012), Enzyme Microb. Technol., 50, 57-64.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.4.99.11 food industry sucrose isomerase activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses. The industrial potential may be further enhanced by selection for variants that do not catabolize the sucrose substrate Rhizobium sp.
5.4.99.11 food industry sucrose isomerase activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses.The industrial potential may be further enhanced by selection for variants that do not catabolize the sucrose substrate Serratia plymuthica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.11 gene mutB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning and expression of wild-type and insertional disruption mutant in Escherichia coli strain BL21 (DE3) Rhizobium sp.
5.4.99.11 gene smuA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning of full-length gene including the periplasmic signal sequence and expression of wild-type and insertional disruption mutant in Escherichia coli strain BL21 (DE3) Serratia plymuthica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.11 sucrose Serratia plymuthica
-
 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?
5.4.99.11 sucrose Rhizobium sp.
-
 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose high product specificity for trehalulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) ?
5.4.99.11 sucrose Serratia plymuthica L0W308
-
 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.11 Rhizobium sp. M1E1F3 i.e. Pseudomonas mesoacidophila MX-45, gene mutB
-
5.4.99.11 Rhizobium sp. M1E1F6 i.e. Pseudomonas mesoacidophila MX-45, gene mutB
-
5.4.99.11 Rhizobium sp. M1E1F7 i.e. Pseudomonas mesoacidophila MX-45, gene mutB
-
5.4.99.11 Serratia plymuthica D0VX20 i.e. Protaminobacter rubrum CBS 574.77, gene smuA
-
5.4.99.11 Serratia plymuthica L0W308 D0VX20 i.e. Protaminobacter rubrum CBS 574.77, gene smuA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.11 recombinant enzyme from Escherichia coli strain BL21(DE3) Serratia plymuthica
5.4.99.11 recombinant enzyme from Escherichia coli strain BL21(DE3) Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.11 sucrose
-
 Serratia plymuthica 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?
5.4.99.11 sucrose
-
 Rhizobium sp. 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?
5.4.99.11 sucrose
-
 Rhizobium sp. 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose high product specificity for trehalulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) ?
5.4.99.11 sucrose
-
 Serratia plymuthica L0W308 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.11 sucrose isomerase
-
 Serratia plymuthica
5.4.99.11 sucrose isomerase
-
 Rhizobium sp.