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Literature summary extracted from

  • de Gonzalo, G.; Rodriguez, C.; Rioz-Martinez, A.; Gotor, V.
    Improvement of the biocatalytic properties of one phenylacetone monooxygenase mutant in hydrophilic organic solvents (2012), Enzyme Microb. Technol., 50, 43-49.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.92 M446G the mutant retains wild type thermostability and produces an altered substrate binding pocket, leading to substantial changes in substrate specificity and enantioselectivity towards sulfides and ketones Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.92 phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
 benzyl acetate + NADP+ + H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.13.92 Methanol methanol induces a significant increase of enzyme activity (up to 5fold), which is optimal at 20% (v/v) Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 PAMO
-
 Thermobifida fusca

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 NADPH
-
 Thermobifida fusca