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Literature summary extracted from

  • Park, H.C.; Park, J.S.; Choi, J.D.; Dabrowski, M.; Atkins, W.M.; Yoon, M.Y.
    Kinetic mechanism of fuculose-1-phosphate aldolase from the hyperthermophilic archaeon Methanococcus jannaschii (2012), Enzyme Microb. Technol., 50, 209-214.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.17 D-psicose 1-phosphate product inhibition is noncompetitive vs. dihydroxyacetone phosphate and competitive vs. DL-glyceraldehyde. Product inhibition is uncompetitive against DHAP at saturated DL-glyceraldehyde Methanocaldococcus jannaschii
4.1.2.17 dihydroxyacetone phosphate
-
 Methanocaldococcus jannaschii
4.1.2.17 DL-glyceraldehyde
-
 Methanocaldococcus jannaschii
4.1.2.17 DL-threose competitive with respect to dihydroxyacetone phosphate and uncompetitive with respect to DL-glyceraldehyde; the dead-end inhibition patterns as assessed by varying the concentration of dihydroxyacetone phosphate at several fixed concentrations of DL-threose is uncompetitive against dihydroxyacetone phosphate at the DL-glyceraldehyde Km concentration, and the dead-end inhibition by varying the concentrations of DL-threose is competitive against DL-glyceraldehyde at the DHAP Km concentration Methanocaldococcus jannaschii
4.1.2.17 L-Tagatose 1-phosphate product inhibition is noncompetitive vs. dihydroxyacetone phosphate and competitive vs. DL-glyceraldehyde. Product inhibition is uncompetitive against DHAP at saturated DL-glyceraldehyde Methanocaldococcus jannaschii
4.1.2.17 trimethyl phosphonoacetate competitive with respect to dihydroxyacetone phosphate and uncompetitive with respect to DL-glyceraldehyde; dead-end inhibition as assessed by varying the dihydroxyacetone phosphate concentration at several fixed concentrations of trimethyl phosphonoacetat: in the direction of the aldol addition reaction, trimethyl phosphonoacetate is competitive vs. dihydroxyacetone phosphate at the DL-glyceraldehyde Km concentration (0.74 mM). In the direction of the aldol addition reaction, trimethyl phosphonoacetate is competitive vs. DL-glyceraldehyde at the dihydroxyacetone phosphate Km concentration (0.091 mM). The dead-end inhibition patterns as assessed by varying the concentration of DL-glyceraldehyde at several fixed concentrations of trimethyl phosphonoacetate: in the direction of the aldol addition reaction, trimethyl phosphonoacetate is competitive vs. DL-glyceraldehyde at the dihydroxyacetone phosphate Km concentration Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.17 0.091
-
 dihydroxyacetone phosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
4.1.2.17 0.091
-
 dihydroxyacetone phosphate pH not specified in the publication, temperature not specified in the publication Methanocaldococcus jannaschii
4.1.2.17 0.743
-
 DL-glyceraldehyde pH not specified in the publication, temperature not specified in the publication Methanocaldococcus jannaschii
4.1.2.17 0.743
-
 D-glyceraldehyde assay with DL-glyceraldehyde, pH and temperature not specified in the publication Methanocaldococcus jannaschii
4.1.2.17 0.743
-
 L-glyceraldehyde assay with DL-glyceraldehyde, pH and temperature not specified in the publication Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.17 Zn2+ contains a catalytically active Zn2+ ion Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.17 Methanocaldococcus jannaschii Q58813
-
-
4.1.2.17 Methanocaldococcus jannaschii DSM 2661 Q58813
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.17 0.0133
-
 in the direction of hydrolysis, pH and temperature not specified in the publication Methanocaldococcus jannaschii
4.1.2.17 0.0256
-
 in the direction of aldol condensation, pH and temperature not specified in the publication Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.17 dihydroxyacetone phosphate + D-glyceraldehyde kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to D-glyceraldehyde Methanocaldococcus jannaschii D-psicose 1-phosphate
-
 ?
4.1.2.17 dihydroxyacetone phosphate + D-glyceraldehyde kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to D-glyceraldehyde Methanocaldococcus jannaschii DSM 2661 D-psicose 1-phosphate
-
 ?
4.1.2.17 dihydroxyacetone phosphate + DL-glyceraldehyde
-
 Methanocaldococcus jannaschii L-tagatose 1-phosphate
-
 r
4.1.2.17 dihydroxyacetone phosphate + DL-glyceraldehyde
-
 Methanocaldococcus jannaschii DSM 2661 L-tagatose 1-phosphate
-
 r
4.1.2.17 dihydroxyacetone phosphate + L-glyceraldehyde kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to L-glyceraldehyde Methanocaldococcus jannaschii L-tagatose 1-phosphate
-
 ?
4.1.2.17 dihydroxyacetone phosphate + L-glyceraldehyde kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to L-glyceraldehyde Methanocaldococcus jannaschii DSM 2661 L-tagatose 1-phosphate
-
 ?
4.1.2.17 dihydroxyacetone phosphate + L-lactaldehyde
-
 Methanocaldococcus jannaschii L-fuculose-1-phosphate
-
 r
4.1.2.17 dihydroxyacetone phosphate + L-lactaldehyde
-
 Methanocaldococcus jannaschii DSM 2661 L-fuculose-1-phosphate
-
 r
4.1.2.17 DL-glycerol 3-phosphate + DL-glyceraldehyde
-
 Methanocaldococcus jannaschii D-psicose 1-phosphate
-
 r
4.1.2.17 additional information rapid equilibrium ordered mechanism in which dihydroxyacetone phosphate is added and bound to the enzyme prior to DL-glyceraldehyde Methanocaldococcus jannaschii ?
-
 ?
4.1.2.17 additional information rapid equilibrium ordered mechanism in which dihydroxyacetone phosphate is added and bound to the enzyme prior to DL-glyceraldehyde Methanocaldococcus jannaschii DSM 2661 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.17 FucA
-
 Methanocaldococcus jannaschii
4.1.2.17 fuculose-1-phosphate aldolase
-
 Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.17 0.01
-
 DL-glyceraldehyde direction of aldol condensation, pH not specified in the publication, temperature not specified in the publication Methanocaldococcus jannaschii
4.1.2.17 0.0123
-
 dihydroxyacetone phosphate direction of aldol condensation, pH not specified in the publication, temperature not specified in the publication Methanocaldococcus jannaschii