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Literature summary extracted from

  • Das, A.; Varma, S.S.; Mularczyk, C.; Meling, D.D.
    Functional investigations of thromboxane synthase (CYP5A1) in lipid bilayers of nanodiscs (2014), ChemBioChem, 15, 892-899.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.5 additional information the catalytic activities of the binary phosphatidylethanolamine/phosphatidylcholine and phosphatidylserine/phosphatidylcholine and ternary phosphatidylcholine/phosphatidylethanolamine/phosphatidylserine are similar and increase relative to the unary phosphatidylcholine system Homo sapiens
5.3.99.5 phosphatidylcholine
-
 Homo sapiens
5.3.99.5 phosphatidylethanolamine
-
 Homo sapiens
5.3.99.5 phosphatidylserine
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.5 expression of C-terminally His-tagged enzyme replacing the first 29 amino acid residues in the N-terminal transmembrane domain with the hydrophilic sequence MAKKTSS for TXAS cDNA in Escherichia coli strain DH5alpha Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.5 endoplasmic reticulum membrane associated Homo sapiens 5789
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.99.5 additional information cyanide binding to substrate-free and substrate-bound CYP5A1 in a detergent-solubilized system and in nanodiscs, binding kinetics, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.5 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate Homo sapiens
-
 (5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.5 recombinant C-terminally His-tagged modified enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.5 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Homo sapiens (5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
-
 ?
5.3.99.5 additional information substrate binding analysis using substrate analogues of PGH2, U44069 and U46619 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.5 CYP5A1
-
 Homo sapiens
5.3.99.5 thromboxane synthase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.99.5 heme
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
5.3.99.5 additional information role of membranes in modulating the thermodynamics and kinetics of enzyme substrate binding, redox potential of enzyme incorporated into lipid bilayers of nanodiscs, overview. Binding of substrate to the enzyme can induce conformational changes in the protein that block small-molecule ligand egress Homo sapiens
5.3.99.5 physiological function the enzyme is a membrane-associated cytochrome P450 that metabolizes the cyclooxygenase product prostaglandin into thromboxane A2, a potent inducer of vasoconstriction and platelet aggregation Homo sapiens