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Literature summary extracted from

  • Khan, K.K.; Halpert, J.R.
    7-Benzyloxyquinoline oxidation by P450eryF A245T: finding of a new fluorescent substrate probe (2002), Chem. Res. Toxicol., 15, 806-814.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.35 A245S significantly increased activity with substrates testosterone and 7-benzyloxyquinoline. Contrary to wild-type, binding to inhibitor ketoconazole follows type II Saccharopolyspora erythraea
1.14.15.35 A245T increased activity with substrate 7-benzyloxyquinoline. Like wild-type, type-II binding to inhibitor ketoconazole Saccharopolyspora erythraea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.35 ketoconazole wild-type and mutant A245S show type-II binding. Mutant A245T shows type-I binding Saccharopolyspora erythraea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.35 0.0103
-
 9-aminophenanthrene wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.9 Saccharopolyspora erythraea
1.14.15.35 0.0312
-
 9-aminophenanthrene mutant A245T, pH 7.5, 37°C, S50 value, Hill coefficient 1.7 Saccharopolyspora erythraea
1.14.15.35 0.261
-
 androstenedione wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.2 Saccharopolyspora erythraea
1.14.15.35 0.277
-
 androstenedione mutant A245T, pH 7.5, 37°C, S50 value, Hill coefficient 1.09 Saccharopolyspora erythraea
1.14.15.35 0.278
-
 testosterone wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.64 Saccharopolyspora erythraea
1.14.15.35 0.326
-
 dehydroepiandrostenedione wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.55 Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.35 Saccharopolyspora erythraea Q00441
-
-
1.14.15.35 Saccharopolyspora erythraea NRRL 2338 Q00441
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.35 9-aminophenanthrene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 9-aminophenanthrene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea NRRL 2338 ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea NRRL 2338 ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 dehydroepiandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 dehydroepiandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea NRRL 2338 ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 additional information enzyme demonstrates cooperative ligand binding and oxidation kinetics Saccharopolyspora erythraea ?
-
 ?
1.14.15.35 additional information enzyme demonstrates cooperative ligand binding and oxidation kinetics Saccharopolyspora erythraea NRRL 2338 ?
-
 ?
1.14.15.35 testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Saccharopolyspora erythraea NRRL 2338 ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.35 cytochrome P450eryF
-
 Saccharopolyspora erythraea
1.14.15.35 eryF
-
 Saccharopolyspora erythraea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.35 cytochrome P450
-
 Saccharopolyspora erythraea