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Literature summary extracted from

  • Wagner, T.; Bellinzoni, M.; Wehenkel, A.; OHare, H.M.; Alzari, P.M.
    Functional plasticity and allosteric regulation of alpha-ketoglutarate decarboxylase in central mycobacterial metabolism (2011), Chem. Biol., 18, 1011-1020.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.71 expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis
4.1.1.71 expressed in Escherichia coli BL21(DE3)pLysS cells Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.71 hanging drop vapor diffusion method, using 39-46.5% (w/v) 2-methyl-2,4-pentanediol, 100 mM HEPES (pH 7.0), 200 mM NaCl Mycobacterium tuberculosis
4.1.1.71 hanging drop vapor diffusion method, using 39-46.5% (w/v) 2-methyl-2,4-pentanediol, 100 mM HEPES (pH 7.0), 200 mM NaCl Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.71 2-oxoglutarate Mycobacterium tuberculosis
-
 succinate semialdehyde + CO2
-
 ?
4.1.1.71 2-oxoglutarate Mycolicibacterium smegmatis
-
 succinate semialdehyde + CO2
-
 ?
4.1.1.71 2-oxoglutarate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 succinate semialdehyde + CO2
-
 ?
4.1.1.71 2-oxoglutarate Mycobacterium tuberculosis H37Rv
-
 succinate semialdehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.71 Mycobacterium tuberculosis
-
-
-
4.1.1.71 Mycobacterium tuberculosis H37Rv
-
-
-
4.1.1.71 Mycolicibacterium smegmatis A0R2B1
-
-
4.1.1.71 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0R2B1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.71 HisTrap column chromatography and Ni-NTA agarose column chromatography Mycobacterium tuberculosis
4.1.1.71 HisTrap column chromatography and Ni-NTA agarose column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.71 2-oxoglutarate
-
 Mycobacterium tuberculosis succinate semialdehyde + CO2
-
 ?
4.1.1.71 2-oxoglutarate
-
 Mycolicibacterium smegmatis succinate semialdehyde + CO2
-
 ?
4.1.1.71 2-oxoglutarate
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 succinate semialdehyde + CO2
-
 ?
4.1.1.71 2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv succinate semialdehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.71 homodimer x-ray crystallography Mycobacterium tuberculosis
4.1.1.71 homodimer x-ray crystallography Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.71 alpha-ketoglutarate decarboxylase
-
 Mycobacterium tuberculosis
4.1.1.71 alpha-ketoglutarate decarboxylase
-
 Mycolicibacterium smegmatis
4.1.1.71 Kgd
-
 Mycobacterium tuberculosis
4.1.1.71 Kgd
-
 Mycolicibacterium smegmatis