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Literature summary extracted from

  • Senarisoy, M.; Canturk, P.; Zencir, S.; Baran, Y.; Topcu, Z.
    Gossypol interferes with both type I and type II topoisomerase activities without generating strand breaks (2013), Cell Biochem. Biophys., 66, 199-204.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.1 spermidine
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 2,2'-bis(8-formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthalene) i.e. gossypol, belongs to the class II compounds which interfere with the catalytic function of topoisomerases without generating strand breaks Bos taurus
5.6.2.1 camptothecin
-
 Bos taurus
5.6.2.2 2,2'-bis(8-formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthalene) i.e. gossypol, belongs to the class II compounds which interfere with the catalytic function of topoisomerases without generating strand breaks Homo sapiens
5.6.2.2 etoposide
-
 Homo sapiens
5.6.2.2 additional information supercoiled pRYG substrate is relaxed with topoisomerase II without a significant effect by DMSO Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.1 Mg2+ required Bos taurus
5.6.2.2 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Bos taurus
-
 calf
-
5.6.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.1 thymus commercial preparation Bos taurus
-
5.6.2.2 additional information recombinant commercial preparation Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 additional information DNA relaxation using supercoiled plasmid DNA pBR322 or pRYG Bos taurus ?
-
 ?
5.6.2.2 additional information decatenation assay with kinetoplast DNA, DNA strand break analysis using supercoiled plasmid DNA pBR322 or pRYG Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.1 Topoisomerase I
-
 Bos taurus
5.6.2.1 type I topoisomerase
-
 Bos taurus
5.6.2.2 Topoisomerase II
-
 Homo sapiens
5.6.2.2 type II topoisomerase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.1 37
-
 assay at Bos taurus
5.6.2.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.1 8
-
 assay at Bos taurus
5.6.2.2 8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP
-
 Homo sapiens