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Literature summary extracted from

  • Fang, H.; Liu, H.; Chen, N.; Zhang, C.; Xie, X.; Xu, Q.
    Site-directed mutagenesis studies on the uridine monophosphate binding sites of feedback inhibition in carbamoyl phosphate synthetase and effects on cytidine production by Bacillus amyloliquefaciens (2013), Can. J. Microbiol., 59, 374-379.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.5 expressed in Escherichia coli BL21 (DE3) cells Bacillus amyloliquefaciens

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 K986I the substrate kinetics (Km for L-glutamine) increases nearly 1.3fold compared to the wild type enzyme Bacillus amyloliquefaciens
6.3.5.5 T941F the substrate kinetics (Km for L-glutamine) increases nearly 1.2fold compared to the wild type enzyme Bacillus amyloliquefaciens
6.3.5.5 T941F/K986I the substrate kinetics (Km for L-glutamine) increases nearly 1.96fold compared to the wild type enzyme Bacillus amyloliquefaciens
6.3.5.5 T941F/T970A/K986I the substrate kinetics (Km for L-glutamine) increases nearly 2.17fold compared to the wild type enzyme Bacillus amyloliquefaciens
6.3.5.5 T970A the substrate kinetics (Km for L-glutamine) increases nearly 1.1fold compared to the wild type enzyme Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP
-
 Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.23
-
 L-glutamine wild type enzyme, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens
6.3.5.5 0.26
-
 L-glutamine mutant enzyme T970A, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens
6.3.5.5 0.28
-
 L-glutamine mutant enzyme T941F, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens
6.3.5.5 0.3
-
 L-glutamine mutant enzyme K986I, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens
6.3.5.5 0.45
-
 L-glutamine mutant enzyme T941F/K986I, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens
6.3.5.5 0.5
-
 L-glutamine mutant enzyme T941F/T970A/K986I, in 50 mM HEPE (pH 7.5), at 37°C Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 40000
-
 2 * 40000, SDS-PAGE Bacillus amyloliquefaciens
6.3.5.5 120000
-
 gel filtration Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 ATP + L-glutamine + HCO3- + H2O Bacillus amyloliquefaciens
-
 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
6.3.5.5 ATP + L-glutamine + HCO3- + H2O Bacillus amyloliquefaciens CYT1
-
 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Bacillus amyloliquefaciens
-
-
-
6.3.5.5 Bacillus amyloliquefaciens CYT1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5 Ni2+-charged HisTrap column column chromatography Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 ATP + L-glutamine + HCO3- + H2O
-
 Bacillus amyloliquefaciens ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?
6.3.5.5 ATP + L-glutamine + HCO3- + H2O
-
 Bacillus amyloliquefaciens CYT1 ADP + phosphate + L-glutamate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 homotrimer 2 * 40000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
6.3.5.5 carbamoyl phosphate synthetase
-
 Bacillus amyloliquefaciens
6.3.5.5 CPS
-
 Bacillus amyloliquefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.5 ATP
-
 Bacillus amyloliquefaciens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.5.5 0.004
-
 wild type enzyme, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens UMP
6.3.5.5 0.0907
-
 mutant enzyme T941F, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens UMP
6.3.5.5 0.1504
-
 mutant enzyme T970A, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens UMP
6.3.5.5 0.2
-
 mutant enzyme K986I, in 50 mM HEPES (pH 7.5), at 37°C Bacillus amyloliquefaciens UMP