EC Number | General Stability | Organism |
---|---|---|
1.5.1.40 | neither high salt or protease inhibitors were effective in stabilizing the activity of the partially purified enzyme | Methanosphaera stadtmanae |
1.5.1.40 | precipitation with (NH4)2SO4 reduces the stability of the enzyme | Methanosphaera stadtmanae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.40 | 0.0077 | - |
reduced coenzyme F420 | pH and temperature not specified in the publication | Methanosphaera stadtmanae | |
1.5.1.40 | 0.0625 | - |
coenzyme F420 | pH and temperature not specified in the publication | Methanosphaera stadtmanae | |
1.5.1.40 | 0.142 | - |
NADPH | pH and temperature not specified in the publication | Methanosphaera stadtmanae | |
1.5.1.40 | 0.37 | - |
NADP+ | pH and temperature not specified in the publication | Methanosphaera stadtmanae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 45000 | - |
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
1.5.1.40 | 50000 | - |
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
1.5.1.40 | 60000 | - |
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
1.5.1.40 | 148000 | - |
gel filtration, native PAGE | Methanosphaera stadtmanae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.40 | reduced coenzyme F420 + NADP+ | Methanosphaera stadtmanae | - |
coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | Methanosphaera stadtmanae DSM 3091 | - |
coenzyme F420 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.40 | Methanosphaera stadtmanae | - |
- |
- |
1.5.1.40 | Methanosphaera stadtmanae DSM 3091 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.40 | - |
Methanosphaera stadtmanae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanosphaera stadtmanae | coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanosphaera stadtmanae DSM 3091 | coenzyme F420 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.40 | trimer | 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.40 | NADP+:F420 oxidoreductase | - |
Methanosphaera stadtmanae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 6 | - |
reduction of coenzyme F420 | Methanosphaera stadtmanae |
1.5.1.40 | 8.5 | - |
reduction of NADP+ | Methanosphaera stadtmanae |