Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Elias, D.A.; Juck, D.F.; Berry, K.A.; Sparling, R.
    Purification of the NADP+:F420 oxidoreductase of Methanosphaera stadtmanae (2000), Can. J. Microbiol., 46, 998-1003.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.40 neither high salt or protease inhibitors were effective in stabilizing the activity of the partially purified enzyme Methanosphaera stadtmanae
1.5.1.40 precipitation with (NH4)2SO4 reduces the stability of the enzyme Methanosphaera stadtmanae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.40 0.0077
-
reduced coenzyme F420 pH and temperature not specified in the publication Methanosphaera stadtmanae
1.5.1.40 0.0625
-
coenzyme F420 pH and temperature not specified in the publication Methanosphaera stadtmanae
1.5.1.40 0.142
-
NADPH pH and temperature not specified in the publication Methanosphaera stadtmanae
1.5.1.40 0.37
-
NADP+ pH and temperature not specified in the publication Methanosphaera stadtmanae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.40 45000
-
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae
1.5.1.40 50000
-
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae
1.5.1.40 60000
-
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae
1.5.1.40 148000
-
gel filtration, native PAGE Methanosphaera stadtmanae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.40 reduced coenzyme F420 + NADP+ Methanosphaera stadtmanae
-
coenzyme F420 + NADPH + H+
-
r
1.5.1.40 reduced coenzyme F420 + NADP+ Methanosphaera stadtmanae DSM 3091
-
coenzyme F420 + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.40 Methanosphaera stadtmanae
-
-
-
1.5.1.40 Methanosphaera stadtmanae DSM 3091
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.40
-
Methanosphaera stadtmanae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.40 reduced coenzyme F420 + NADP+
-
Methanosphaera stadtmanae coenzyme F420 + NADPH + H+
-
r
1.5.1.40 reduced coenzyme F420 + NADP+
-
Methanosphaera stadtmanae DSM 3091 coenzyme F420 + NADPH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.5.1.40 trimer 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae

Synonyms

EC Number Synonyms Comment Organism
1.5.1.40 NADP+:F420 oxidoreductase
-
Methanosphaera stadtmanae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.40 6
-
reduction of coenzyme F420 Methanosphaera stadtmanae
1.5.1.40 8.5
-
reduction of NADP+ Methanosphaera stadtmanae