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Literature summary extracted from

  • Karan, R.; Capes, M.D.; DasSarma, P.; DasSarma, S.
    Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi (2013), BMC Biotechnol., 13, 3.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expressed in the genetically tractable haloarchaeon Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter Halorubrum lacusprofundi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 ethanol 10% v/v, about 40% loss of activity Halorubrum lacusprofundi
3.2.1.23 isoamyl alcohol 10% v/v, about 35% loss of activity Halorubrum lacusprofundi
3.2.1.23 methanol 10% v/v, about 5% loss of activity Halorubrum lacusprofundi
3.2.1.23 n-butanol 10% v/v, about 35% loss of activity Halorubrum lacusprofundi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 KCl maximal activity in either 4 M NaCl or KCl Halorubrum lacusprofundi
3.2.1.23 NaCl maximal activity in either 4 M NaCl or KCl Halorubrum lacusprofundi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 78060
-
 x * 78060, calculated from sequence Halorubrum lacusprofundi
3.2.1.23 100000
-
 x * 100000, SDS-PAGE Halorubrum lacusprofundi

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.23 Ethanol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi
3.2.1.23 isoamyl alcohol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi
3.2.1.23 Methanol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi
3.2.1.23 n-Butanol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Halorubrum lacusprofundi B9LW38
-
-
3.2.1.23 Halorubrum lacusprofundi DSM 5036 B9LW38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Halorubrum lacusprofundi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 110.8
-
 pH 6.0, 25°C Halorubrum lacusprofundi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Halorubrum lacusprofundi 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Halorubrum lacusprofundi DSM 5036 2-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 100000, SDS-PAGE Halorubrum lacusprofundi
3.2.1.23 ? x * 78060, calculated from sequence Halorubrum lacusprofundi

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 Hlac_2868 locus name Halorubrum lacusprofundi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Halorubrum lacusprofundi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 35 60 35°C: about 55% of maximal activity, 60°C: about 80% of maximal activity Halorubrum lacusprofundi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
-
 Halorubrum lacusprofundi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 6 7.5 pH 6.0: about 60% of maximal activity, pH 7.5: about 40% of maximal activity Halorubrum lacusprofundi