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Literature summary extracted from
- Cloning, expression and characterization of a phosphoglucomutase/phosphomannomutase from sphingan-producing Sphingomonas sanxanigenens (2013), Biotechnol. Lett., 35, 1265-1270.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.4.2.2 | food industry | the enzyme is involved in production of sphingans, extracellular polysaccharides used as thickeners, emulsifiers and gelling agents, its overexpression increases the sphingan production of the transformed cell | Sphingomonas sanxanigenens |
| 5.4.2.8 | food industry | the enzyme is involved in production of sphingans, extracellular polysaccharides used as thickeners, emulsifiers and gelling agents, its overexpression increases the sphingan production of the transformed cell | Sphingomonas sanxanigenens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.2 | gene pgmG, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha | Sphingomonas sanxanigenens |
| 5.4.2.8 | gene pgmG, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha | Sphingomonas sanxanigenens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.2 | 0.2 | 1 | alpha-D-glucose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens |  |
| 5.4.2.8 | 0.68 | - |
alpha-D-mannose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.2 | 49800 | - |
x * 49800, about, sequence calculation | Sphingomonas sanxanigenens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.2 | alpha-D-glucose 1-phosphate | Sphingomonas sanxanigenens | - |
D-glucose 6-phosphate | - |
? | |
| 5.4.2.2 | alpha-D-glucose 1-phosphate | Sphingomonas sanxanigenens NX02 | - |
D-glucose 6-phosphate | - |
? | |
| 5.4.2.2 | additional information | Sphingomonas sanxanigenens | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | ? | - |
? | |
| 5.4.2.2 | additional information | Sphingomonas sanxanigenens NX02 | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | ? | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | Sphingomonas sanxanigenens | - |
D-mannose 6-phosphate | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | Sphingomonas sanxanigenens NX02 | - |
D-mannose 6-phosphate | - |
? | |
| 5.4.2.8 | additional information | Sphingomonas sanxanigenens | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | ? | - |
? | |
| 5.4.2.8 | additional information | Sphingomonas sanxanigenens NX02 | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.2 | Sphingomonas sanxanigenens | M1T754 | gene pgmG | - |
| 5.4.2.2 | Sphingomonas sanxanigenens NX02 | M1T754 | gene pgmG | - |
| 5.4.2.8 | Sphingomonas sanxanigenens | M1T754 | gene pgmG | - |
| 5.4.2.8 | Sphingomonas sanxanigenens NX02 | M1T754 | gene pgmG | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.2 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sphingomonas sanxanigenens |
| 5.4.2.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sphingomonas sanxanigenens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.2 | alpha-D-glucose 1-phosphate | - |
Sphingomonas sanxanigenens | D-glucose 6-phosphate | - |
? | |
| 5.4.2.2 | alpha-D-glucose 1-phosphate | - |
Sphingomonas sanxanigenens NX02 | D-glucose 6-phosphate | - |
? | |
| 5.4.2.2 | additional information | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | Sphingomonas sanxanigenens | ? | - |
? | |
| 5.4.2.2 | additional information | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | Sphingomonas sanxanigenens NX02 | ? | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | - |
Sphingomonas sanxanigenens | D-mannose 6-phosphate | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | - |
Sphingomonas sanxanigenens NX02 | D-mannose 6-phosphate | - |
? | |
| 5.4.2.8 | additional information | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | Sphingomonas sanxanigenens | ? | - |
? | |
| 5.4.2.8 | additional information | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | Sphingomonas sanxanigenens NX02 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.2.2 | ? | x * 49800, about, sequence calculation | Sphingomonas sanxanigenens |
| 5.4.2.8 | ? | x ** 49800, about, sequence calculation | Sphingomonas sanxanigenens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.2 | PGM/PMM | - |
Sphingomonas sanxanigenens |
| 5.4.2.2 | PgmG | - |
Sphingomonas sanxanigenens |
| 5.4.2.2 | phosphoglucomutase/phosphomannomutase | - |
Sphingomonas sanxanigenens |
| 5.4.2.8 | PGM/PMM | - |
Sphingomonas sanxanigenens |
| 5.4.2.8 | PgmG | - |
Sphingomonas sanxanigenens |
| 5.4.2.8 | phosphoglucomutase/phosphomannomutase | - |
Sphingomonas sanxanigenens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.2 | 10.77 | - |
alpha-D-glucose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
| 5.4.2.8 | 2.28 | - |
alpha-D-mannose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.2 | 7.6 | - |
assay at | Sphingomonas sanxanigenens |
| 5.4.2.8 | 7.6 | - |
assay at | Sphingomonas sanxanigenens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.2.2 | physiological function | the enzyme is involved in biosynthesis of sphingans, extracellular polysaccharides | Sphingomonas sanxanigenens |
| 5.4.2.8 | physiological function | the enzyme is involved in biosynthesis of sphingans, extracellular polysaccharides | Sphingomonas sanxanigenens |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.2 | 51.27 | - |
alpha-D-glucose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
| 5.4.2.8 | 3.35 | - |
alpha-D-mannose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
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