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Literature summary extracted from

  • Kim, H.J.; Yeom, S.J.; Kim, K.; Rhee, S.; Kim, D.; Oh, D.K.
    Mutational analysis of the active site residues of a D-psicose 3-epimerase from Agrobacterium tumefaciens (2010), Biotechnol. Lett., 32, 261-268.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.B12 E156D mutant exhibits 63% of the wild-type activity Agrobacterium tumefaciens
5.1.3.B12 R215K mutant exhibits 25% of the wild-type activity Agrobacterium tumefaciens
5.1.3.B12 W112A mutant enzyme exhibits no detectable activity Agrobacterium tumefaciens
5.1.3.B12 W112F mutant enzyme displays 19% of the wild type enzyme activity. kcat/Km for D-fructose is 100fold lower than wild-type value, kcat/Km fur D-psicose is 84fold lower than wild-type value Agrobacterium tumefaciens
5.1.3.B12 W112H mutant enzyme displays 65% of the wild type enzyme activity Agrobacterium tumefaciens
5.1.3.B12 W112Y mutant enzyme displays 54% of the wild type enzyme activity Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.B12 1 3 D-psicose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 33
-
 D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 37
-
 D-psicose pH 8.0, 45°C, mutant enzyme R215K Agrobacterium tumefaciens
5.1.3.B12 48
-
 D-psicose pH 8.0, 45°C, mutant enzyme E156D Agrobacterium tumefaciens
5.1.3.B12 100
-
 D-fructose pH 8.0, 45°C, mutant enzyme D183E Agrobacterium tumefaciens
5.1.3.B12 100
-
 D-psicose pH 8.0, 45°C, mutant enzyme W112F Agrobacterium tumefaciens
5.1.3.B12 139
-
 D-fructose pH 8.0, 45°C, mutant enzyme E156D Agrobacterium tumefaciens
5.1.3.B12 270
-
 D-fructose pH 8.0, 45°C, mutant enzyme W112F Agrobacterium tumefaciens
5.1.3.B12 309
-
 D-fructose pH 8.0, 45°C, mutant enzyme R215K Agrobacterium tumefaciens
5.1.3.B12 427
-
 D-fructose pH 8.0, 45°C, mutant enzyme H209A Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.B12 Mn2+ the distances between the catalytic residues (Glu150 and Glu244) and Mn2+ are critical to the catalysis, and the negative charges of the metal-binding residues are important for interaction with metal ion Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.B12 Agrobacterium tumefaciens A9CH28
-
-
5.1.3.B12 Agrobacterium tumefaciens ATCC 33970 A9CH28
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.B12 D-fructose
-
 Agrobacterium tumefaciens D-psicose
-
 r
5.1.3.B12 D-fructose
-
 Agrobacterium tumefaciens ATCC 33970 D-psicose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.B12 45
-
 assay at Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.B12 0.029
-
 D-fructose pH 8.0, 45°C, mutant enzyme D183E Agrobacterium tumefaciens
5.1.3.B12 4.9
-
 D-fructose pH 8.0, 45°C, mutant enzyme R215K Agrobacterium tumefaciens
5.1.3.B12 7.7
-
 D-fructose pH 8.0, 45°C, mutant enzyme H209A Agrobacterium tumefaciens
5.1.3.B12 17
-
 D-fructose pH 8.0, 45°C, mutant enzyme E156D Agrobacterium tumefaciens
5.1.3.B12 23
-
 D-psicose pH 8.0, 45°C, mutant enzyme R215K Agrobacterium tumefaciens
5.1.3.B12 26
-
 D-fructose pH 8.0, 45°C, mutant enzyme W112F Agrobacterium tumefaciens
5.1.3.B12 44
-
 D-psicose pH 8.0, 45°C, mutant enzyme W112F Agrobacterium tumefaciens
5.1.3.B12 46
-
 D-psicose pH 8.0, 45°C, mutant enzyme E156D Agrobacterium tumefaciens
5.1.3.B12 341
-
 D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 478
-
 D-psicose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.B12 8
-
 assay at Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.B12 0.00029
-
 D-fructose pH 8.0, 45°C, mutant enzyme D183E Agrobacterium tumefaciens
5.1.3.B12 0.015
-
 D-fructose pH 8.0, 45°C, mutant enzyme R215K Agrobacterium tumefaciens
5.1.3.B12 0.018
-
 D-fructose pH 8.0, 45°C, mutant enzyme H209A Agrobacterium tumefaciens
5.1.3.B12 0.1
-
 D-fructose pH 8.0, 45°C, mutant enzyme W112F Agrobacterium tumefaciens
5.1.3.B12 0.12
-
 D-fructose pH 8.0, 45°C, mutant enzyme E156D Agrobacterium tumefaciens
5.1.3.B12 0.44
-
 D-psicose pH 8.0, 45°C, mutant enzyme W112F Agrobacterium tumefaciens
5.1.3.B12 0.62
-
 D-psicose pH 8.0, 45°C, mutant enzyme R215K Agrobacterium tumefaciens
5.1.3.B12 0.96
-
 D-psicose pH 8.0, 45°C, mutant enzyme E156D Agrobacterium tumefaciens
5.1.3.B12 10
-
 D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 37
-
 D-psicose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens