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Literature summary extracted from

  • Seo, M.J.; Nam, Y.D.; Lee, S.Y.; Park, S.L.; Yi, S.H.; Lim, S.I.
    Expression and characterization of a glutamate decarboxylase from Lactobacillus brevis 877G producing gamma-aminobutyric acid (2013), Biosci. Biotechnol. Biochem., 77, 853-856.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gad, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Levilactobacillus brevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 AgNO3
-
Levilactobacillus brevis
4.1.1.15 CuCl2
-
Levilactobacillus brevis
4.1.1.15 KI
-
Levilactobacillus brevis
4.1.1.15 Na2SO4
-
Levilactobacillus brevis
4.1.1.15 ZnCl2
-
Levilactobacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 3.6
-
L-glutamate pH 5.2, 45°C, recombinant enzyme Levilactobacillus brevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 CaCl2 potent activation Levilactobacillus brevis
4.1.1.15 additional information no effect on activity by NaCl, MgCl2, and MnCl2 Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 50000
-
x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
4.1.1.15 53522
-
x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Levilactobacillus brevis
-
4-aminobutanoate + CO2
-
?
4.1.1.15 L-glutamate Levilactobacillus brevis 877G
-
4-aminobutanoate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Levilactobacillus brevis K4HXK6 isolated from traditional Korean fermented food kimchi, gene gad
-
4.1.1.15 Levilactobacillus brevis 877G K4HXK6 isolated from traditional Korean fermented food kimchi, gene gad
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Levilactobacillus brevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.15 additional information strain 877G, isolated from traditional Korean fermented food kimchi, shows very high gamma-aminobutanoate production Levilactobacillus brevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
Levilactobacillus brevis 4-aminobutanoate + CO2
-
?
4.1.1.15 L-glutamate
-
Levilactobacillus brevis 877G 4-aminobutanoate + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 ? x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 LbGAD
-
Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 45
-
recombinant enzyme Levilactobacillus brevis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.15 0.286
-
L-glutamate pH 5.2, 45°C, recombinant enzyme Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 5.2
-
recombinant enzyme Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate residue K279 is essential for cofactor binding Levilactobacillus brevis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.15 Levilactobacillus brevis sequence calculation
-
5.4

General Information

EC Number General Information Comment Organism
4.1.1.15 additional information residues T215 and D246 are involved in catalysis Levilactobacillus brevis