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Literature summary extracted from

  • Innocenti, A.; Zimmerman. S.; Ferry, J.G.; Scozzafava, A.; Supuran, C.T.
    Carbonic anhydrase inhibitors. Inhibition of the zinc and cobalt gamma-class enzyme from the archaeon Methanosarcina thermophila with anions (2004), Bioorg. Med. Chem. Lett., 14, 3327-3331.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 azide weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 Br-
-
 Methanosarcina thermophila
4.2.1.1 Carbonate weak inhibitor of the Zn(II)-substituted enzyme, best inhibitor of the Co(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 CN- best anion inhibitors for Zn2+-substituted enzyme are cyanate and hydrogen sulfide Methanosarcina thermophila
4.2.1.1 CNO-
-
 Methanosarcina thermophila
4.2.1.1 CO32-
-
 Methanosarcina thermophila
4.2.1.1 Cyanate best inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 cyanide
-
 Methanosarcina thermophila
4.2.1.1 HCO3-
-
 Methanosarcina thermophila
4.2.1.1 HS- best anion inhibitors for Zn2+-substituted enzyme are cyanate and hydrogen sulfide Methanosarcina thermophila
4.2.1.1 HSO3-
-
 Methanosarcina thermophila
4.2.1.1 hydrogen sulfide best inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 hydrogen sulfite weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 hydrogencarbonate good inhibitor of the Co(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 I-
-
 Methanosarcina thermophila
4.2.1.1 additional information fluoride, chloride, and sulfate do not inhibit the Zn2+-substituted enzyme and the Co2+-substituted enzyme appreciably; Zn(II)- and Co(II)-substituted enzymes show a very different behavior against anion inhibitors.The best Zn-Cam anion inhibitors are hydrogen sulfide and cyanate whereas thiocyanate, azide, carbonate, nitrite, and hydrogensulfite are weaker inhibitors. The inhibition of the Zn(II)-substituted enzyme by sulfide is puzzling since Cam is proposed to be located outside the cytoplasmic membrane where it is exposed to high levels of sulfide in the anaerobic environments. The best inhibitors of the Co(II)-substituted enzyme is carbonate, followed by nitrate and hydrogencarbonate. Fluoride, chloride, and sulfate do not inhibit either Zn(II)- or Co(II)-substituted enzyme appreciably up to concentrations of 200 mM. This lack of inhibition may represent an adaptation to marine environments high in chloride and sulfate Methanosarcina thermophila
4.2.1.1 N3-
-
 Methanosarcina thermophila
4.2.1.1 nitrate good inhibitor of the Co(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 nitrite weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
4.2.1.1 NO2-
-
 Methanosarcina thermophila
4.2.1.1 NO3-
-
 Methanosarcina thermophila
4.2.1.1 SCN-
-
 Methanosarcina thermophila
4.2.1.1 thiocyanate weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 additional information the metal ion in the enzyme isolated directly from Methanosarcina thermophila is not been determined since only enzyme overproduced in Escherichia coli is characterized Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanosarcina thermophila
-
-
-
4.2.1.1 Methanosarcina thermophila P40881
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 20
-
 assay at Methanosarcina thermophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.009
-
 CO32- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 0.009
-
 Carbonate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 0.05
-
 HS- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 0.05
-
 hydrogen sulfide Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 0.09
-
 NO3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 0.09
-
 CNO- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 0.09
-
 nitrate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 0.09
-
 Cyanate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 0.1
-
 HCO3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 0.1
-
 hydrogencarbonate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 0.68
-
 CN- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 0.68
-
 cyanide Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 1.8
-
 HSO3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 1.8
-
 hydrogen sulfite Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 2
-
 HS- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 2
-
 hydrogen sulfide Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 4.7
-
 CNO- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 4.7
-
 Cyanate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 4.9
-
 N3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 4.9
-
 azide Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 5.3
-
 I- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 5.3
-
 I- Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 5.8
-
 N3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 5.8
-
 azide Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 6.1
-
 SCN- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 6.1
-
 thiocyanate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 6.7
-
 CO32- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 6.7
-
 Carbonate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 6.8
-
 NO2- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 6.8
-
 nitrite Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 7
-
 SCN- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 7
-
 thiocyanate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 7.3
-
 NO2- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 7.3
-
 nitrite Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 11.7
-
 HSO3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 11.7
-
 hydrogen sulfite Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 22.2
-
 Br- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 22.2
-
 Br- Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 36.5
-
 NO3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 36.5
-
 nitrate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 42
-
 HCO3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 42
-
 hydrogencarbonate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 51.5
-
 CN- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 51.5
-
 cyanide Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 160
-
 Br- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 160
-
 I- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
4.2.1.1 160
-
 Br- Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.2.1.1 160
-
 I- Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila