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Literature summary extracted from
- The cysteine 703 to isoleucine or histidine mutation of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae generates an iridal-type triterpenoid (2012), Biochimie, 94, 2376-2381.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | synthesis | engineering ERG7 for producing biological active agents is promising | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | C703D | site-directed mutagenesis, the mutant shows unaltered product spectrum compared to the wild-type enzyme | Saccharomyces cerevisiae |
| 5.4.99.7 | C703G | site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, but not as diverse as mutants C703I and C703H | Saccharomyces cerevisiae |
| 5.4.99.7 | C703H | site-directed mutagenesis, the mutant generates an unusual truncated bicyclic rearranged intermediate, (8R,9R,10R)-polypoda-5,13E,17E,21-tetraen-3beta-ol, related to iridal-skeleton triterpenoid. Numerous oxidosqualene-cyclized truncated intermediates, including tricyclic, unrearranged tetracyclic with 17alpha/beta exocyclic hydrocarbon side chain, rearranged tetracyclic, and chair-chair-chair tricyclic intermediates, are also isolated from the mutant | Saccharomyces cerevisiae |
| 5.4.99.7 | C703I | site-directed mutagenesis, the mutant generates an unusual truncated bicyclic rearranged intermediate, (8R,9R,10R)-polypoda-5,13E,17E,21-tetraen-3beta-ol, related to iridal-skeleton triterpenoid. Numerous oxidosqualene-cyclized truncated intermediates, including tricyclic, unrearranged tetracyclic with 17alpha/beta exocyclic hydrocarbon side chain, rearranged tetracyclic, and chair-chair-chair tricyclic intermediates, are also isolated from the mutant | Saccharomyces cerevisiae |
| 5.4.99.7 | C703N | site-directed mutagenesis, the mutant shows unaltered product spectrum compared to the wild-type enzyme | Saccharomyces cerevisiae |
| 5.4.99.7 | C703S | site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, but not as diverse as mutants C703I and C703H | Saccharomyces cerevisiae |
| 5.4.99.7 | C703T | site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, but not as diverse as mutants C703I and C703H | Saccharomyces cerevisiae |
| 5.4.99.7 | C703V | site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, but not as diverse as mutants C703I and C703H | Saccharomyces cerevisiae |
| 5.4.99.7 | F699A/C703I | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| 5.4.99.7 | F699M/C703I | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| 5.4.99.7 | F699T/C703I | site-directed mutagenesis, different oxidosqualene-cyclized truncated intermediates, including tricyclic, unrearranged tetracyclic with 17alpha/beta exocyclic hydrocarbon side chain, rearranged tetracyclic, and chair-chair-chair tricyclic intermediates, are isolated from the mutant | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.7 | Saccharomyces cerevisiae | - |
gene ERG7 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | ERG7 | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | OSC | - |
Saccharomyces cerevisiae |
| 5.4.99.7 | Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.7 | physiological function | functional role of the Cys703 residue in stabilizing the bicyclic C-8 cation and the rearranged intermediate or interacting with Phe699 | Saccharomyces cerevisiae |
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