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Literature summary extracted from

  • Hayashi, M.; Kobayashi, K.; Esaki, H.; Konno, H.; Akaji, K.; Tazuya, K.; Yamada, K.; Nakabayashi, T.; Nosaka, K.
    Enzymatic and structural characterization of an archaeal thiamin phosphate synthase (2014), Biochim. Biophys. Acta, 1844, 803-809.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.3 expression in Escherichia coli Pyrobaculum calidifontis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.3 H341L no activity Pyrobaculum calidifontis
2.5.1.3 R320M activity is 1% compared to wild-type enzyme Pyrobaculum calidifontis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine 0.1 mM, 6.3% inhibition Pyrobaculum calidifontis
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate 0.1 mM, 18.2% inhibition Pyrobaculum calidifontis
2.5.1.3 5-(2-hydroxyethyl)-4-methylthiazole 0.1 mM, 12.3% inhibition Pyrobaculum calidifontis
2.5.1.3 ATP 0.1 mM, 12.8% inhibition Pyrobaculum calidifontis
2.5.1.3 diphosphate 0.1 mM, 66% inhibition Pyrobaculum calidifontis
2.5.1.3 thiamin 0.1 mM, 17.3% inhibition Pyrobaculum calidifontis
2.5.1.3 thiamin phosphate 0.1 mM, 59% inhibition Pyrobaculum calidifontis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.3 Mg2+ an absolute requirement of Mg2+ for the activity is replaced to some extent by Co2+, Ca2+ or Zn2+. When Mg2+ is replaced by Mn2+, Ni2+ or Fe2+, however, the activity is much lower or not detected Pyrobaculum calidifontis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole Pyrobaculum calidifontis thiamin biosynthesis diphosphate + thiamine phosphate
-
 ?
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole Pyrobaculum calidifontis JCM 11548 thiamin biosynthesis diphosphate + thiamine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.3 Pyrobaculum calidifontis A3MSR0
-
-
2.5.1.3 Pyrobaculum calidifontis JCM 11548 A3MSR0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.3
-
 Pyrobaculum calidifontis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.3 additional information
-
 specific activity of 2.1 nmol thiamin phosphate formed/mg min Pyrobaculum calidifontis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
-
 Pyrobaculum calidifontis diphosphate + thiamine phosphate
-
 ?
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole thiamin biosynthesis Pyrobaculum calidifontis diphosphate + thiamine phosphate
-
 ?
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
-
 Pyrobaculum calidifontis JCM 11548 diphosphate + thiamine phosphate
-
 ?
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole thiamin biosynthesis Pyrobaculum calidifontis JCM 11548 diphosphate + thiamine phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.3 thiamin phosphate synthase
-
 Pyrobaculum calidifontis
2.5.1.3 ThiN protein
-
 Pyrobaculum calidifontis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.3 100
-
 assay at Pyrobaculum calidifontis
2.5.1.3 110
-
-
 Pyrobaculum calidifontis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.3 80 120 activity increases with an increase in temperature from 80°C to 120°C, and the highest activity is observed around 110°C. The activity at 100°C is about 75% of the highest activity Pyrobaculum calidifontis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.3 additional information
-
 addition of 0.5 M NaCl to the enzyme solution does not improve the thermostability Pyrobaculum calidifontis
2.5.1.3 100
-
 5 min, 20% loss of activity. 15 min, 50% loss of activity. 2 h, 90% loss of activity Pyrobaculum calidifontis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.3 7.5
-
 assay at Pyrobaculum calidifontis
2.5.1.3 8.5
-
-
 Pyrobaculum calidifontis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.3 7.5 8.5 the optimum pH is pH 8.5, and the activity at pH 7.5 is about 60% of that at pH 8.5 Pyrobaculum calidifontis