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Literature summary extracted from

  • Bonete, M.J.; Perez-Pomares, F.; Ferrer, J.; Camacho, M.L.
    NAD-glutamate dehydrogenase from Halobacterium halobium: inhibition and activation by TCA intermediates and amino acids (1996), Biochim. Biophys. Acta, 1289, 14-24.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 glycine
-
 Halobacterium salinarum
1.4.1.2 L-alanine
-
 Halobacterium salinarum
1.4.1.2 L-arginine
-
 Halobacterium salinarum
1.4.1.2 L-asparagine
-
 Halobacterium salinarum
1.4.1.2 L-aspartic acid
-
 Halobacterium salinarum
1.4.1.2 L-cysteine
-
 Halobacterium salinarum
1.4.1.2 L-glutamine
-
 Halobacterium salinarum
1.4.1.2 L-histidine
-
 Halobacterium salinarum
1.4.1.2 L-isoleucine
-
 Halobacterium salinarum
1.4.1.2 L-leucine
-
 Halobacterium salinarum
1.4.1.2 L-lysine
-
 Halobacterium salinarum
1.4.1.2 L-methionine
-
 Halobacterium salinarum
1.4.1.2 L-phenylalanine
-
 Halobacterium salinarum
1.4.1.2 L-serine
-
 Halobacterium salinarum
1.4.1.2 L-threonine
-
 Halobacterium salinarum
1.4.1.2 L-tryptophan
-
 Halobacterium salinarum
1.4.1.2 L-tyrosine
-
 Halobacterium salinarum
1.4.1.2 L-valine
-
 Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 2-oxoglutarate non-competitive versus L-glutamate, competitive versus NAD+ Halobacterium salinarum
1.4.1.2 adipate
-
 Halobacterium salinarum
1.4.1.2 D-glutamate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
1.4.1.2 fumarate non-competitive versus L-glutamate, competitive versus NAD+ Halobacterium salinarum
1.4.1.2 Glutarate uncompetitive versus L-glutamate, competitive versus NAD+ Halobacterium salinarum
1.4.1.2 malate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
1.4.1.2 additional information GTP, ATP, ADP and AMP do not affect the activity Halobacterium salinarum
1.4.1.2 NADP+ non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
1.4.1.2 oxaloacetate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum
1.4.1.2 succinate non-competitive versus L-glutamate, non-competitive versus NAD+ Halobacterium salinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+ Halobacterium salinarum
-
 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Halobacterium salinarum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Halobacterium salinarum 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 NAD-GDH
-
 Halobacterium salinarum
1.4.1.2 NAD-glutamate dehydrogenase
-
 Halobacterium salinarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.2 40
-
 assay at Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 8
-
 assay at Halobacterium salinarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.2 0.78
-
 NADP+ pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 1
-
 2-oxoglutarate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 1.54
-
 fumarate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 2.5
-
 2-oxoglutarate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 3
-
 D-glutamate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 3
-
 Glutarate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 3 7 oxaloacetate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 3 4 D-glutamate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 5
-
 NADP+ pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 5.5
-
 2-oxoglutarate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 5.7
-
 fumarate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 8.3
-
 succinate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 8.4
-
 fumarate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 9
-
 NADP+ pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 10
-
 2-oxoglutarate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 18.7
-
 D-glutamate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 20
-
 fumarate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 22
-
 malate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 24
-
 succinate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 26
-
 oxaloacetate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 28
-
 succinate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 33
-
 succinate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 35
-
 adipate pH 8.0, 40°C, Ki(slope), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 53
-
 Glutarate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 64
-
 malate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 65
-
 malate pH 8.0, 40°C, Ki(intercept), at 2.5 mM NAD+ Halobacterium salinarum
1.4.1.2 66
-
 malate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 174
-
 oxaloacetate pH 8.0, 40°C, Ki(intercept), at 120 mM L-glutamate Halobacterium salinarum
1.4.1.2 187
-
 oxaloacetate pH 8.0, 40°C, Ki(slope), at 120 mM L-glutamate Halobacterium salinarum