BRENDA - Enzyme Database show

Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics

Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Osipov, E.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.; Biochemistry 79, 54-61 (2014)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
2-mercaptoethanol
2 mM, 2fold increase in activity
Acidilobus saccharovorans
3.5.1.19
dithiothreitol
2 mM, 2.1fold increase in activity
Acidilobus saccharovorans
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.B15
expressed in Escherichia coli
Acidilobus saccharovorans
3.5.1.19
expressed in Escherichia coli
Acidilobus saccharovorans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.B15
Zn2+
-
Acidilobus saccharovorans
3.5.1.19
EDTA
10 mM, 57% loss of activity
Acidilobus saccharovorans
3.5.1.19
Fe3+
1 mM, 94% inhibition
Acidilobus saccharovorans
3.5.1.19
NaCl
500 mM, 12% inhibition
Acidilobus saccharovorans
3.5.1.19
Zn2+
5 mM, complete inactivation
Acidilobus saccharovorans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.B15
0.41
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
0.18
-
nicotinamide
pH and temperature not specified in the publication
Acidilobus saccharovorans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.B15
Fe2+
the enzyme contains Fe2+ in the active site
Acidilobus saccharovorans
3.5.1.19
Fe2+
the enzyme contains Fe2+ in the active site
Acidilobus saccharovorans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.B15
46400
-
-
Acidilobus saccharovorans
3.5.1.19
44600
-
-
Acidilobus saccharovorans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.B15
Acidilobus saccharovorans
D9Q1R5
-
-
3.5.1.19
Acidilobus saccharovorans
D9Q1R5
-
-
3.5.1.19
Acidilobus saccharovorans DSM 16705
D9Q1R5
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.B15
pyrazinamide + H2O
the enzyme also catalyzes the hydrolysis of nicotinamide
727053
Acidilobus saccharovorans
pyrazinoic acid + NH3
-
-
-
?
3.5.1.B15
pyrazinamide + H2O
the enzyme also catalyzes the hydrolysis of nicotinamide
727053
Acidilobus saccharovorans DSM 16705
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
the enzyme also catalyzes the hydrolysis of pyrazinamide
727053
Acidilobus saccharovorans
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
the enzyme also catalyzes the hydrolysis of pyrazinamide
727053
Acidilobus saccharovorans DSM 16705
nicotinate + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.B15
homodimer
-
Acidilobus saccharovorans
3.5.1.19
homodimer
-
Acidilobus saccharovorans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.B15
90
-
-
Acidilobus saccharovorans
3.5.1.19
90
-
-
Acidilobus saccharovorans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.19
65
98
65°C: about 50% of maximal activity, 98°C: about 80% of maximal activity
Acidilobus saccharovorans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.B15
60
-
t1/2: 180 min
Acidilobus saccharovorans
3.5.1.B15
80
-
t1/2: 35 min
Acidilobus saccharovorans
3.5.1.19
60
-
t1/2: 180 min
Acidilobus saccharovorans
3.5.1.19
80
-
t1/2: 35 min
Acidilobus saccharovorans
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.B15
135.7
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
76.9
-
nicotinamide
pH and temperature not specified in the publication
Acidilobus saccharovorans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.B15
7.5
-
-
Acidilobus saccharovorans
3.5.1.19
7.5
-
-
Acidilobus saccharovorans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
2-mercaptoethanol
2 mM, 2fold increase in activity
Acidilobus saccharovorans
3.5.1.19
dithiothreitol
2 mM, 2.1fold increase in activity
Acidilobus saccharovorans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.B15
expressed in Escherichia coli
Acidilobus saccharovorans
3.5.1.19
expressed in Escherichia coli
Acidilobus saccharovorans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.B15
Zn2+
-
Acidilobus saccharovorans
3.5.1.19
EDTA
10 mM, 57% loss of activity
Acidilobus saccharovorans
3.5.1.19
Fe3+
1 mM, 94% inhibition
Acidilobus saccharovorans
3.5.1.19
NaCl
500 mM, 12% inhibition
Acidilobus saccharovorans
3.5.1.19
Zn2+
5 mM, complete inactivation
Acidilobus saccharovorans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.B15
0.41
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
0.18
-
nicotinamide
pH and temperature not specified in the publication
Acidilobus saccharovorans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.B15
Fe2+
the enzyme contains Fe2+ in the active site
Acidilobus saccharovorans
3.5.1.19
Fe2+
the enzyme contains Fe2+ in the active site
Acidilobus saccharovorans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.B15
46400
-
-
Acidilobus saccharovorans
3.5.1.19
44600
-
-
Acidilobus saccharovorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.B15
pyrazinamide + H2O
the enzyme also catalyzes the hydrolysis of nicotinamide
727053
Acidilobus saccharovorans
pyrazinoic acid + NH3
-
-
-
?
3.5.1.B15
pyrazinamide + H2O
the enzyme also catalyzes the hydrolysis of nicotinamide
727053
Acidilobus saccharovorans DSM 16705
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
the enzyme also catalyzes the hydrolysis of pyrazinamide
727053
Acidilobus saccharovorans
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
the enzyme also catalyzes the hydrolysis of pyrazinamide
727053
Acidilobus saccharovorans DSM 16705
nicotinate + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.B15
homodimer
-
Acidilobus saccharovorans
3.5.1.19
homodimer
-
Acidilobus saccharovorans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.B15
90
-
-
Acidilobus saccharovorans
3.5.1.19
90
-
-
Acidilobus saccharovorans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.19
65
98
65°C: about 50% of maximal activity, 98°C: about 80% of maximal activity
Acidilobus saccharovorans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.B15
60
-
t1/2: 180 min
Acidilobus saccharovorans
3.5.1.B15
80
-
t1/2: 35 min
Acidilobus saccharovorans
3.5.1.19
60
-
t1/2: 180 min
Acidilobus saccharovorans
3.5.1.19
80
-
t1/2: 35 min
Acidilobus saccharovorans
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.B15
135.7
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
76.9
-
nicotinamide
pH and temperature not specified in the publication
Acidilobus saccharovorans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.B15
7.5
-
-
Acidilobus saccharovorans
3.5.1.19
7.5
-
-
Acidilobus saccharovorans
General Information
EC Number
General Information
Commentary
Organism
3.5.1.19
physiological function
the enzyme is involved in the maintenance of NAD+ homeostasis and in the NAD+ salvage pathway of most prokaryotes
Acidilobus saccharovorans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.19
physiological function
the enzyme is involved in the maintenance of NAD+ homeostasis and in the NAD+ salvage pathway of most prokaryotes
Acidilobus saccharovorans
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.B15
331
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
427
-
nicotinamide
pH and temperature not specified in the publication
Acidilobus saccharovorans
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.B15
331
-
Pyrazinamide
pH 7.5, 60°C
Acidilobus saccharovorans
3.5.1.19
427
-
nicotinamide
pH and temperature not specified in the publication
Acidilobus saccharovorans