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Literature summary extracted from

  • Kumar, G.; Frantom, P.A.
    Evolutionarily distinct versions of the multidomain enzyme alpha-isopropylmalate synthase share discrete mechanisms of V-type allosteric regulation (2014), Biochemistry, 53, 4847-4856.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 EDTA 1 mM, 96% decrease in activity Methanocaldococcus jannaschii
2.3.3.13 L-leucine partially inhibited by L-leucine in a V-type manner Methanocaldococcus jannaschii
2.3.3.13 MgCl2 20 mM decreases activity Methanocaldococcus jannaschii
2.3.3.13 MnCl2 20 mM decreases activity Methanocaldococcus jannaschii
2.3.3.13 ZnCl2 20 mM decreases activity Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.13 0.07
-
acetyl-CoA pH 7.8, 37°C Methanocaldococcus jannaschii
2.3.3.13 0.09
-
3-methyl-2-oxobutanoate pH 7.8, 37°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.13 KCl 100 mM, activity is increased 1.5fold Methanocaldococcus jannaschii
2.3.3.13 additional information the enzyme is active in the absence of exogenous monovalent or divalent metals. Addition of 1 mM EDTA results in a 96% decrease in activity, consistent with the requirement for divalent metals and suggesting that the enzyme is copurified with a divalent metal Methanocaldococcus jannaschii
2.3.3.13 NaCl 100 mM, activity is increased 1.5fold Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.13 230000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Methanocaldococcus jannaschii the hydrolytic step is rate-determining (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Methanocaldococcus jannaschii DSM 2661 the hydrolytic step is rate-determining (2S)-2-isopropylmalate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Methanocaldococcus jannaschii Q58595
-
-
2.3.3.13 Methanocaldococcus jannaschii DSM 2661 Q58595
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13
-
Methanocaldococcus jannaschii

Storage Stability

EC Number Storage Stability Organism
2.3.3.13 -20°C, concentrated protein stored in 20% glycerol Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O the hydrolytic step is rate-determining Methanocaldococcus jannaschii (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O the hydrolytic step is rate-determining Methanocaldococcus jannaschii DSM 2661 (2S)-2-isopropylmalate + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 IPMS
-
Methanocaldococcus jannaschii
2.3.3.13 leuA
-
Methanocaldococcus jannaschii
2.3.3.13 Mj1195 locus name Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.13 37
-
assay at Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.13 90
-
the enzyme retains full activity after incubation in water for 15 min Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 330
-
acetyl-CoA pH 7.8, 37°C Methanocaldococcus jannaschii
2.3.3.13 330
-
3-methyl-2-oxobutanoate pH 7.8, 37°C Methanocaldococcus jannaschii
2.3.3.13 450
-
acetyl-CoA pH 7.8, 55°C Methanocaldococcus jannaschii
2.3.3.13 450
-
3-methyl-2-oxobutanoate pH 7.8, 55°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.13 7.8
-
assay at Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.13 0.00016
-
L-leucine pH 7.8, 37°C Methanocaldococcus jannaschii