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Literature summary extracted from

  • Wang, S.; Thomas, K.; Schramm, V.L.
    Catalytic site cooperativity in dimeric methylthioadenosine nucleosidase (2014), Biochemistry, 53, 1527-1535.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.9 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus
3.2.2.16 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus
3.2.2.16 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
3.2.2.16 expressed in Escherichia coli BL21(DE3) cells Vibrio cholerae
3.2.2.30 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.16 sitting drop vapor diffusion method Vibrio cholerae
3.2.2.16 sitting drop vapor diffusion method, using Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.9 E173Q inactive Staphylococcus aureus
3.2.2.9 F104C/C186S the mutations give an enzyme with steady-state kinetic properties similar to the native enzyme Staphylococcus aureus
3.2.2.16 A113P the mutant shows reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
3.2.2.16 A113P/V153I the mutant shows reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
3.2.2.16 A113P/V153I/R158G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
3.2.2.16 E173Q inactive Staphylococcus aureus
3.2.2.16 F104C/C186S the mutant shows reduced activity compared to the wild type Staphylococcus aureus
3.2.2.16 V153I the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
3.2.2.30 E173Q inactive Staphylococcus aureus
3.2.2.30 F104C/C186S the mutations give an enzyme with steady-state kinetic properties similar to the native enzyme Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.9 methylthio-DADMe-immucillin-A i.e. (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol Staphylococcus aureus
3.2.2.16 (3R,4S)-1-[9-deazaadenin-(9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine
-
 Staphylococcus aureus
3.2.2.16 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Escherichia coli
3.2.2.16 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Vibrio cholerae
3.2.2.30 methylthio-DADMe-immucillin-A i.e. (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.16 0.000061
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.000139
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Escherichia coli
3.2.2.16 0.000202
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.000262
-
 S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.000262
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.00043
-
 S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.0009
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Staphylococcus aureus
3.2.2.16 0.0029
-
 S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.9 S-adenosyl-L-homocysteine + H2O Staphylococcus aureus
-
 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.16 S-adenosyl-L-homocysteine + H2O Staphylococcus aureus
-
 S-D-ribosyl-L-homocysteine + adenine
-
 ir
3.2.2.16 S-methyl-5'-thioadenosine + H2O Staphylococcus aureus
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O Escherichia coli
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O Vibrio cholerae
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O Staphylococcus aureus
-
 dehypoxanthine futalosine + adenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.9 Staphylococcus aureus
-
-
-
3.2.2.16 Escherichia coli P0AF12
-
-
3.2.2.16 Staphylococcus aureus Q99TQ0
-
-
3.2.2.16 Vibrio cholerae A5F5R2
-
-
3.2.2.30 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.9 Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus
3.2.2.16 Ni-NTA column chromatography Escherichia coli
3.2.2.16 Ni-NTA column chromatography Vibrio cholerae
3.2.2.16 Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus
3.2.2.30 Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Staphylococcus aureus S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.16 S-adenosyl-L-homocysteine + H2O
-
 Staphylococcus aureus S-D-ribosyl-L-homocysteine + adenine
-
 ir
3.2.2.16 S-methyl-5'-thioadenosine + H2O
-
 Staphylococcus aureus S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O
-
 Escherichia coli S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O
-
 Vibrio cholerae S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.30 5,5'-dithiobis-2-nitrobenzoic acid + H2O
-
 Staphylococcus aureus ?
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O
-
 Staphylococcus aureus dehypoxanthine futalosine + adenine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.9 homodimer
-
 Staphylococcus aureus
3.2.2.16 homodimer
-
 Staphylococcus aureus
3.2.2.30 homodimer
-
 Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.2.2.9 methylthioadenosine nucleosidase
-
 Staphylococcus aureus
3.2.2.9 MTAN
-
 Staphylococcus aureus
3.2.2.16 5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase
-
 Escherichia coli
3.2.2.16 5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase
-
 Vibrio cholerae
3.2.2.16 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Staphylococcus aureus
3.2.2.16 methylthioadenosine nucleosidase
-
 Escherichia coli
3.2.2.16 methylthioadenosine nucleosidase
-
 Vibrio cholerae
3.2.2.16 MTAN
-
 Staphylococcus aureus
3.2.2.16 MTAN
-
 Escherichia coli
3.2.2.16 MTAN
-
 Vibrio cholerae
3.2.2.30 methylthioadenosine nucleosidase
-
 Staphylococcus aureus
3.2.2.30 MTAN
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.16 0.0079
-
 S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.074
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.11
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.17
-
 S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.18
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 0.32
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Escherichia coli
3.2.2.16 7.2
-
 S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C Staphylococcus aureus
3.2.2.16 10.2
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.16 18
-
 S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 200
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 400
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 700
-
 S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 2300
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Escherichia coli
3.2.2.16 2480
-
 S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C Staphylococcus aureus
3.2.2.16 3000
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Vibrio cholerae
3.2.2.16 11300
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Staphylococcus aureus