BRENDA - Enzyme Database show

3-Phosphoglycerate is an allosteric activator of pyruvate kinase from the hyperthermophilic archaeon Pyrobaculum aerophilum

Solomons, J.T.; Johnsen, U.; Schönheit, P.; Davies, C.; Biochemistry 52, 5865-5875 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
3-phosphoglycerate
allosteric activator. Regulation of the enzyme by a carboxylate molecule rather than a sugar phosphate may reflect a step in the evolution of glycolysis that predates the dominance of sugars in metabolism
Pyrobaculum aerophilum
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.40
-
Pyrobaculum aerophilum
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.1.40
hanging-drop vapor diffusion method at 18°C, crystal structure is determined to 2.2 A resolution
Pyrobaculum aerophilum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.6
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
Pyrobaculum aerophilum
-
3-phospho-D-glycerate + H+ + reduced ferredoxin
-
-
ir
2.7.1.40
ATP + pyruvate
Pyrobaculum aerophilum
the enzyme catalyzes the final step of glycolysis
ADP + phosphoenolpyruvate
-
-
?
2.7.1.40
ATP + pyruvate
Pyrobaculum aerophilum DSM 7523
the enzyme catalyzes the final step of glycolysis
ADP + phosphoenolpyruvate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.6
Pyrobaculum aerophilum
-
-
-
2.7.1.40
Pyrobaculum aerophilum
Q8ZYE0
-
-
2.7.1.40
Pyrobaculum aerophilum DSM 7523
Q8ZYE0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
-
Pyrobaculum aerophilum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.6
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
727026
Pyrobaculum aerophilum
3-phospho-D-glycerate + H+ + reduced ferredoxin
-
-
-
ir
2.7.1.40
ATP + pyruvate
-
727026
Pyrobaculum aerophilum
ADP + phosphoenolpyruvate
-
-
-
?
2.7.1.40
ATP + pyruvate
the enzyme catalyzes the final step of glycolysis
727026
Pyrobaculum aerophilum
ADP + phosphoenolpyruvate
-
-
-
?
2.7.1.40
ATP + pyruvate
-
727026
Pyrobaculum aerophilum DSM 7523
ADP + phosphoenolpyruvate
-
-
-
?
2.7.1.40
ATP + pyruvate
the enzyme catalyzes the final step of glycolysis
727026
Pyrobaculum aerophilum DSM 7523
ADP + phosphoenolpyruvate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
3-phosphoglycerate
allosteric activator. Regulation of the enzyme by a carboxylate molecule rather than a sugar phosphate may reflect a step in the evolution of glycolysis that predates the dominance of sugars in metabolism
Pyrobaculum aerophilum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
-
Pyrobaculum aerophilum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.1.40
hanging-drop vapor diffusion method at 18°C, crystal structure is determined to 2.2 A resolution
Pyrobaculum aerophilum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.6
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
Pyrobaculum aerophilum
-
3-phospho-D-glycerate + H+ + reduced ferredoxin
-
-
ir
2.7.1.40
ATP + pyruvate
Pyrobaculum aerophilum
the enzyme catalyzes the final step of glycolysis
ADP + phosphoenolpyruvate
-
-
?
2.7.1.40
ATP + pyruvate
Pyrobaculum aerophilum DSM 7523
the enzyme catalyzes the final step of glycolysis
ADP + phosphoenolpyruvate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
-
Pyrobaculum aerophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.6
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
727026
Pyrobaculum aerophilum
3-phospho-D-glycerate + H+ + reduced ferredoxin
-
-
-
ir
2.7.1.40
ATP + pyruvate
-
727026
Pyrobaculum aerophilum
ADP + phosphoenolpyruvate
-
-
-
?
2.7.1.40
ATP + pyruvate
the enzyme catalyzes the final step of glycolysis
727026
Pyrobaculum aerophilum
ADP + phosphoenolpyruvate
-
-
-
?
2.7.1.40
ATP + pyruvate
-
727026
Pyrobaculum aerophilum DSM 7523
ADP + phosphoenolpyruvate
-
-
-
?
2.7.1.40
ATP + pyruvate
the enzyme catalyzes the final step of glycolysis
727026
Pyrobaculum aerophilum DSM 7523
ADP + phosphoenolpyruvate
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.7.1.40
physiological function
the enzyme catalyzes the final step of glycolysis
Pyrobaculum aerophilum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.40
physiological function
the enzyme catalyzes the final step of glycolysis
Pyrobaculum aerophilum