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Literature summary extracted from

  • Bowsher, C.G.; Eyres, L.M.; Gummadova, J.O.; Hothi, P.; McLean, K.J.; Munro, A.W.; Scrutton, N.S.; Hanke, G.T.; Sakakibara, Y.; Hase, T.
    Identification of N-terminal regions of wheat leaf ferredoxin NADP+ oxidoreductase important for interactions with ferredoxin (2011), Biochemistry, 50, 1778-1787.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 additional information study on impact of N-terminal truncation on interaction with ferredoxin using deletions of up to 25 amino acids. Similar kcat values for cytochrome c reduction are measured for all but the most truncated pFNRII[N-5]DEGV which contains a truncated N-terminal domain beginning after the KISKK domain. The longer forms of isoform FNRII bind more strongly to a ferredoxin affinity column than the shorter forms. Mutants bearing lysine-to-glutamine mutations in the KISKK domain are affected in binding to the ferredoxin column Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.00205
-
 ferricytochrome c mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 0.00312
-
 ferricytochrome c wild-type, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 0.0035
-
 ferricytochrome c mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 0.00999
-
 ferricytochrome c mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 0.0103
-
 ferricytochrome c mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 0.0104
-
 ferricytochrome c mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 0.01103
-
 ferricytochrome c mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Triticum aestivum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.2 leaf
-
 Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 ferricytochrome c2 + NADPH
-
 Triticum aestivum 2 ferrocytochrome c2 + NADP+ + H+
-
 ?
1.18.1.2 ferricytochrome c + NADP+
-
 Triticum aestivum ferrocytochrome c + NADPH + H+
-
 r
1.18.1.2 ferrocytochrome c + NADP+
-
 Triticum aestivum ferricytochrome c + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 FNRII
-
 Triticum aestivum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 38.1
-
 reduced flavodoxin mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 45.4
-
 reduced flavodoxin mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 65
-
 reduced flavodoxin mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 66.3
-
 reduced flavodoxin mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 104
-
 reduced flavodoxin wild-type, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 128.2
-
 reduced flavodoxin mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 139.4
-
 reduced flavodoxin mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication Triticum aestivum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 3700
-
 ferricytochrome c mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 4520
-
 ferricytochrome c mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 12640
-
 ferricytochrome c mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 12830
-
 ferricytochrome c mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 18940
-
 ferricytochrome c mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 31710
-
 ferricytochrome c mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication Triticum aestivum
1.18.1.2 33330
-
 ferricytochrome c wild-type, pH 7.5, temperature not specified in the publication Triticum aestivum