EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.4 | expression in Escherichia coli BL21 | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.4 | adenylosuccinate | competitive inhibitor of IMP, noncompetitive inhibitor of GTP and aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | AMP | competitive with respect to IMP and noncompetitive with GTP and aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | D-fructose 1,6 bisphosphate | competitive with IMP and noncompetitive against GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | GDP | competitive inhibitor of GTP, noncompetitive inhibitor with IMP and aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | GMP | competitive with respect to GTP and noncompetitive with IMP and aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | IMP | inhibits the enzyme activity at subsaturating GTP concentrations | Methanocaldococcus jannaschii | |
6.3.4.4 | N-formyl-N-hydroxyglycine | i.e. hadacidin. competitive inhibitor of aspartate, showed uncompetitive inhibition with IMP and GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | phosphate | potent inhibitor, biphasic inhibition, competitive with IMP and noncompetitive with GTP | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.4 | 0.0426 | - |
GTP | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0755 | - |
IMP | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 1.079 | - |
L-aspartate | pH 6.0, 70°C | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.4 | Mg2+ | requires Mg2+ for catalysis with maximum activity obtained at 15 mM magnesium acetate | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.4 | 37855 | - |
2 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl | Methanocaldococcus jannaschii |
6.3.4.4 | 37855 | - |
4 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.4 | Methanocaldococcus jannaschii | Q57981 | - |
- |
6.3.4.4 | Methanocaldococcus jannaschii DSM 2661 | Q57981 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.4 | - |
Methanocaldococcus jannaschii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.4.4 | GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP | rapid equilibrium random AB steady-state ordered C kinetic mechanism | Methanocaldococcus jannaschii |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.4.4 | -20°C, stable without any decrease in activity for at least 6 months | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.4 | GTP + IMP + L-aspartate | - |
Methanocaldococcus jannaschii | GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP | i.e. adenylosuccinate | ? | |
6.3.4.4 | GTP + IMP + L-aspartate | - |
Methanocaldococcus jannaschii DSM 2661 | GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP | i.e. adenylosuccinate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.4 | dimer | 2 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl | Methanocaldococcus jannaschii |
6.3.4.4 | tetramer | 4 * 37855, MALDI mass spectrometric analysis, equilibrium mixture of dimers and tetramers with the tetramer being the catalytically active form. The tetramer dissociates into dimers with a minor increase in ionic strength of the buffer, while the dimer is extremely stable and does not dissociate even at 1.2 M NaCl | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.4 | AdSS | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.4.4 | 70 | - |
asasy at | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.4 | 0.45 | - |
GTP | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.45 | - |
L-aspartate | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.45 | - |
IMP | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 4.2 | - |
GTP | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 4.2 | - |
L-aspartate | pH 6.0, 70°C | Methanocaldococcus jannaschii | |
6.3.4.4 | 4.2 | - |
IMP | pH 6.0, 70°C | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.4.4 | 6 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.4 | 0.007 | - |
GDP | pH 6.0, 70°C, competitive inhibition of GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0094 | - |
adenylosuccinate | pH 6.0, 70°C, competitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0105 | - |
N-formyl-N-hydroxyglycine | pH 6.0, 70°C, competitive inhibition of asparate | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0137 | - |
GMP | pH 6.0, 70°C, noncompetitive inhibition of GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0264 | - |
N-formyl-N-hydroxyglycine | pH 6.0, 70°C, uncompetitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0269 | - |
N-formyl-N-hydroxyglycine | pH 6.0, 70°C, uncompetitive inhibition of GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0426 | - |
adenylosuccinate | pH 6.0, 70°C, noncompetitive inhibition of aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0429 | - |
adenylosuccinate | pH 6.0, 70°C, noncompetitive inhibition of GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0582 | - |
AMP | pH 6.0, 70°C, competitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0821 | - |
GDP | pH 6.0, 70°C, noncompetitive inhibition of aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.0822 | - |
GDP | pH 6.0, 70°C, noncompetitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.11 | - |
GMP | pH 6.0, 70°C, noncompetitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.12 | - |
GMP | pH 6.0, 70°C, noncompetitive inhibition of asparate | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.324 | - |
AMP | pH 6.0, 70°C, noncompetitive inhibition of aspartate | Methanocaldococcus jannaschii | |
6.3.4.4 | 0.402 | - |
AMP | pH 6.0, 70°C, noncompetitive inhibition of GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | 1.5 | - |
D-fructose 1,6 bisphosphate | pH 6.0, 70°C, competitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 1.9 | - |
phosphate | pH 6.0, 70°C, competitive inhibition of IMP | Methanocaldococcus jannaschii | |
6.3.4.4 | 4.8 | - |
D-fructose 1,6 bisphosphate | pH 6.0, 70°C, competitive inhibition of GTP | Methanocaldococcus jannaschii | |
6.3.4.4 | 8.3 | - |
phosphate | pH 6.0, 70°C, competitive inhibition of GTP | Methanocaldococcus jannaschii |