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Literature summary extracted from
- L-Cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide (2005), Biochemistry, 44, 1659-1670.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.28 | dithiothreitol | the air-inactivated enzyme is activated by reaction with Fe2+ and dithiothreitol in the absence of air | Methanocaldococcus jannaschii |  |
| 4.4.1.28 | methyl viologen | 4 mM, 40% stimulation | Methanocaldococcus jannaschii | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.4.1.28 | expression in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.4.1.28 | C25A | mutation reduces the catalytic efficiency and thermal stability of the enzyme | Methanocaldococcus jannaschii |
| 4.4.1.28 | C282A | mutation reduces the catalytic efficiency and thermal stability of the enzyme | Methanocaldococcus jannaschii |
| 4.4.1.28 | C322S | mutation reduces the catalytic efficiency and thermal stability of the enzyme | Methanocaldococcus jannaschii |
| 4.4.1.28 | C329S | mutation reduces the catalytic efficiency and thermal stability of the enzyme | Methanocaldococcus jannaschii |
| 4.4.1.28 | C332A/C329A | complete loss of activity | Methanocaldococcus jannaschii |
| 4.4.1.28 | D323N | about 45% loss of specific activity | Methanocaldococcus jannaschii |
| 4.4.1.28 | H139N | about 10% loss of specific activity | Methanocaldococcus jannaschii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.28 | EDTA | 7.1 mM, 70% inhibition | Methanocaldococcus jannaschii | |
| 4.4.1.28 | hydroxylamine | 34.5 mM, 12% inhibition | Methanocaldococcus jannaschii | |
| 4.4.1.28 | iodoacetamide | 0.42 mM, half-life: 12 min. 17 mM, preincubation completely abolishes activity | Methanocaldococcus jannaschii | |
| 4.4.1.28 | N-ethylmaleimide | 0.42 mM, half-life: 8 min | Methanocaldococcus jannaschii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.28 | 1.78 | - |
L-cysteine | 65°C, pH 6.6, wild-type enzyme | Methanocaldococcus jannaschii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.28 | Fe2+ | the air-inactivated enzyme is activated by reaction with Fe2+ and dithiothreitol in the absence of air | Methanocaldococcus jannaschii | |
| 4.4.1.28 | Iron-sulfur cluster | the air-inactivated enzyme contains 3 mol of iron per subunit | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.28 | 43000 | - |
3 * 43000, SDS-PAGE | Methanocaldococcus jannaschii |
| 4.4.1.28 | 135000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.28 | L-cysteine + H2O | Methanocaldococcus jannaschii | possible involvement in iron-sulfur center biosynthesis | sulfide + NH3 + pyruvate | - |
? | |
| 4.4.1.28 | L-cysteine + H2O | Methanocaldococcus jannaschii DSM 2661 | possible involvement in iron-sulfur center biosynthesis | sulfide + NH3 + pyruvate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.28 | Methanocaldococcus jannaschii | Q58431 | - |
- |
| 4.4.1.28 | Methanocaldococcus jannaschii DSM 2661 | Q58431 | - |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.4.1.28 | activity is completely lost by incubating the native protein solution in air for 15 min prior to the incubation | Methanocaldococcus jannaschii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.28 | - |
Methanocaldococcus jannaschii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.4.1.28 | 2-aminoprop-2-enoate = 2-iminopropanoate | (1b), spontaneous | Methanocaldococcus jannaschii | |
| 4.4.1.28 | 2-iminopropanoate + H2O = pyruvate + NH3 | (1c), spontaneous | Methanocaldococcus jannaschii | |
| 4.4.1.28 | L-cysteine + H2O = sulfide + NH3 + pyruvate | overall reaction | Methanocaldococcus jannaschii | |
| 4.4.1.28 | L-cysteine = 2-aminoprop-2-enoate + sulfide | (1a) | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.28 | 0.22 | - |
65°C, pH 6.6, mutant enzyme C25A | Methanocaldococcus jannaschii |
| 4.4.1.28 | 2.8 | - |
65°C, pH 6.6, mutant enzyme C282A | Methanocaldococcus jannaschii |
| 4.4.1.28 | 6.5 | - |
65°C, pH 6.6, mutant enzyme C322S | Methanocaldococcus jannaschii |
| 4.4.1.28 | 6.8 | - |
65°C, pH 6.6, mutant enzyme C329S | Methanocaldococcus jannaschii |
| 4.4.1.28 | 30 | - |
65°C, pH 6.6, mutant enzyme D323N | Methanocaldococcus jannaschii |
| 4.4.1.28 | 49 | - |
65°C, pH 6.6, mutant enzyme H139N | Methanocaldococcus jannaschii |
| 4.4.1.28 | 55.8 | - |
65°C, pH 6.6, wild-type enzyme | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.28 | L-cysteine + H2O | possible involvement in iron-sulfur center biosynthesis | Methanocaldococcus jannaschii | sulfide + NH3 + pyruvate | - |
? | |
| 4.4.1.28 | L-cysteine + H2O | the enzyme is very specific for L-cysteine, with no activity being detected with D-cysteine, L-homocysteine, 3-mercaptopropionic acid (cysteine without the amino group), cysteamine (cysteine without the carboxylic acid), or mercaptolactate (the hydroxyl analogue of cysteine) | Methanocaldococcus jannaschii | sulfide + NH3 + pyruvate | - |
? | |
| 4.4.1.28 | L-cysteine + H2O | possible involvement in iron-sulfur center biosynthesis | Methanocaldococcus jannaschii DSM 2661 | sulfide + NH3 + pyruvate | - |
? | |
| 4.4.1.28 | L-cysteine + H2O | the enzyme is very specific for L-cysteine, with no activity being detected with D-cysteine, L-homocysteine, 3-mercaptopropionic acid (cysteine without the amino group), cysteamine (cysteine without the carboxylic acid), or mercaptolactate (the hydroxyl analogue of cysteine) | Methanocaldococcus jannaschii DSM 2661 | sulfide + NH3 + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.28 | trimer | 3 * 43000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.4.1.28 | L-cysteine desulfhydrase | - |
Methanocaldococcus jannaschii |
| 4.4.1.28 | MJ1025 | locus name | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.28 | 90 | - |
10 min, 22% loss of activity, mutant enzyme C278A | Methanocaldococcus jannaschii |
| 4.4.1.28 | 90 | - |
10 min, 65% loss of activity, mutant enzyme C318S | Methanocaldococcus jannaschii |
| 4.4.1.28 | 90 | - |
10 min, 80% loss of activity, mutant enzyme C325S | Methanocaldococcus jannaschii |
| 4.4.1.28 | 90 | - |
10 min, no loss of activity, wild-type enzyme | Methanocaldococcus jannaschii |
| 4.4.1.28 | 100 | - |
10 min, 80% loss of activity, wild-type enzyme | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.28 | 39.6 | - |
L-cysteine | 65°C, pH 6.6, wild-type enzyme | Methanocaldococcus jannaschii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.28 | 7.6 | - |
sharp pH-optiumum | Methanocaldococcus jannaschii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.28 | 6.5 | 8.5 | activities at pH 6.5 and 8.5 are less than 20% of that observed at pH 7.6 | Methanocaldococcus jannaschii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.28 | additional information | both the isolated and recombinant enzymes are devoid of pyridoxal 5'-phosphate | Methanocaldococcus jannaschii |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.4.1.28 | physiological function | possible involvement in iron-sulfur center biosynthesis | Methanocaldococcus jannaschii |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.28 | 22.2 | - |
L-cysteine | 65°C, pH 6.6, wild-type enzyme | Methanocaldococcus jannaschii | |
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