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Literature summary extracted from

  • Chaudhuri, B.N.; Lange, S.C.; Myers, R.S.; Davisson, V.J.; Smith, J.L.
    Toward understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerolphosphate synthase: crystal structures of a ternary complex and the free enzyme (2003), Biochemistry, 42, 7003-7012.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.B2 structures of a complex of the substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate and imidazole glycerol phosphate synthase covalently modified by the glutamine analogue acvicin at 2.5 A, apoenzyme at 2.4 A, and imidazole glycerol phosphate synthase inactivated by the glutamine analogue 6-diazo-5-oxo-L-norleucine at 2.5 A. Comparison of the free, acivicin-, and 6-diazo-5-oxo-L-norleucine-inactivated forms of the glutaminase active site reveals structural changes relevant to activation. The N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate-bound and -free forms of the enzyme shed light on flexible parts of the molecule with potential roles in crosstalk between active sites Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.B2 6-diazo-5-oxo-L-norleucine
-
 Saccharomyces cerevisiae
4.3.1.B2 acivicin
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae the imidazole glycerol phosphate synthase catalyzes formation of the imidazole ring in histidine biosynthesis L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.B2 Saccharomyces cerevisiae P33734
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate the imidazole glycerol phosphate synthase catalyzes formation of the imidazole ring in histidine biosynthesis Saccharomyces cerevisiae L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate the enzyme is also a glutamine amidotransferase, which produces ammonia in a glutaminase active site and channels it through a 30 A internal tunnel to a cyclase active site Saccharomyces cerevisiae L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
4.3.1.B2 NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.1.B2 IGPS
-
 Saccharomyces cerevisiae