EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.35 | Saccharopolyspora erythraea | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.35 | (9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | hydroxylation at a rate approximately 2fold lower than the natural substrate 6-deoxyerythronolide B | Saccharopolyspora erythraea | (9R)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.35 | (9S)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | hydroxylation at a rate approximately equal to the natural substrate 6-deoxyerythronolide B | Saccharopolyspora erythraea | (9S)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.35 | (9S)-9-deoxo-9-hydroxy-8,8a-deoxyoleandolide + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | substrate is identical to 6-deoxyerythronolide B except for the presence of a C13 methyl group. Hydroxylation at a rate approximately 4fold lower than the natural substrate 6-deoxyerythronolide B | Saccharopolyspora erythraea | 6-hydroxy-8,8a-deoxyoleandolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.35 | 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.35 | 8,8a-deoxyoleandolide + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | substrate is identical to 6-deoxyerythronolide B except for the presence of a C13 methyl group. Hydroxylation at a rate approximately 4fold lower than the natural substrate 6-deoxyerythronolide B | Saccharopolyspora erythraea | 6-hydroxy-8,8a-deoxyoleandolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.35 | additional information | the ethyl substituent at the 13-position of 6-deoxyerythronolide B is important in the affinity of the substrate for the enzyme, possibly by interacting with a hydrophobic active-site residue. Residue Leu76 may be in a position to interact with the C-13 ethyl group | Saccharopolyspora erythraea | ? | - |
? |