Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shafiee, A.; Hutchinson, C.R.
    Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus) (1987), Biochemistry, 26, 6204-6210.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.35 0.0115
-
(9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B pH 7.3, 35°C Saccharopolyspora erythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.35 44000
-
PAGE Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.35 Saccharopolyspora erythraea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.35 isolation of two forms, P-4501 and P-45011, by hydroxylapatite chromatography, having a P-450 content of 17.5 and 15.2 nmol/mg of protein, respectively Saccharopolyspora erythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.35 (9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
Saccharopolyspora erythraea (9R)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
1.14.15.35 (9S)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
Saccharopolyspora erythraea (9S)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
1.14.15.35 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
Saccharopolyspora erythraea erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.15.35 monomer 1 * 44000, SDS-PAGE Saccharopolyspora erythraea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.35 cytochrome P450 enzyme contains cytochrome P-450 in the low spin state with absorption maxima at 416, 532, and 565 nm, respectively Saccharopolyspora erythraea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.15.35 Saccharopolyspora erythraea isoelectric focusing
-
4.6