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Literature summary extracted from

  • Jahic, H.; Liu, C.F.; Thresher, J.; Livchak, S.; Wang, H.; Ehmann, D.E.
    The kinetic mechanism of S. pneumoniae DNA ligase and inhibition by adenosine-based antibacterial compounds (2012), Biochem. Pharmacol., 84, 654-660.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 L75F L75F mutant is as catalytically efficient as wild-type, athough the NAD Km is slightly elevated compared to wild-type, it is still likely well below the concentration of NAD in the bacterium. IC50 for inhibitors elevated compared to wild-type Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
-
 Streptococcus pneumoniae
6.5.1.2 (2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
-
 Streptococcus pneumoniae
6.5.1.2 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
-
 Streptococcus pneumoniae
6.5.1.2 deoxyribonucleotide
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.2 0.000028
-
 deoxyribonucleotide co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae
6.5.1.2 0.000028
-
 deoxyribonucleotide wild-type, 25°C, pH 7.5 Streptococcus pneumoniae
6.5.1.2 0.004
-
 NAD+ wild-type, 25°C, pH 7.5 Streptococcus pneumoniae
6.5.1.2 0.026
-
 NAD+ mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + deoxyribonucleotide Streptococcus pneumoniae
-
 AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + deoxyribonucleotide
-
 Streptococcus pneumoniae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 NAD-dependent DNA ligase
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.2 0.27
-
 deoxyribonucleotide co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae
6.5.1.2 0.27
-
 deoxyribonucleotide co-substrate: NAD+, wild-type, 25°C, pH 7.5 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.5.1.2 0.000051
-
 deoxyribonucleotide wild-type, 25°C, pH 7.5 Streptococcus pneumoniae
6.5.1.2 0.000065
-
 deoxyribonucleotide co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.5.1.2 0.00004
-
 wild-type, 25°C, pH 7.5 Streptococcus pneumoniae 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
6.5.1.2 0.0001
-
 wild-type, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
6.5.1.2 0.0002
-
 wild-type, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
6.5.1.2 0.01
-
 mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
6.5.1.2 0.012
-
 mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
6.5.1.2 0.03
-
 mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol