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Literature summary extracted from

  • Calvo, A.; Pey, A.; Miranda-Vizuete, A.; Døskeland, A.; Martinez, A.
    Divergence in enzyme regulation between Caenorhabditis elegans and human tyrosine hydroxylase, the key enzyme in the synthesis of dopamine (2011), Biochem. J., 434, 133-141.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expressed in Escherichia coli BL21(DE3) pLysS cells Homo sapiens
1.14.16.2 expressed in Escherichia coli BL21(DE3) pLysS cells Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 3,4-dihydroxy-L-phenylalanine product inhibition Caenorhabditis elegans
1.14.16.2 3,4-dihydroxy-L-phenylalanine product inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Homo sapiens the enzyme shows high substrate specificity for L-tyrosine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Caenorhabditis elegans the enzyme shows high substrate specificity for L-tyrosine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Caenorhabditis elegans N2 Bristol the enzyme shows high substrate specificity for L-tyrosine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Caenorhabditis elegans
-
-
-
1.14.16.2 Caenorhabditis elegans N2 Bristol
-
-
-
1.14.16.2 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.2 phosphoprotein the enzyme is phosphorylated at Ser35 by cAMP-dependent protein kinase Caenorhabditis elegans
1.14.16.2 phosphoprotein the enzyme is phosphorylated at Ser40 by cAMP-dependent protein kinase Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 GST-Sepharose column chromatography Homo sapiens
1.14.16.2 GST-Sepharose column chromatography Caenorhabditis elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 0.555
-
 with 0.05 mM L-tyrosine, at pH 7.0 and 25°C Homo sapiens
1.14.16.2 68800
-
 with 1 mM L-tyrosine, at pH 7.0 and 25°C Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 the enzyme shows high substrate specificity for L-tyrosine Homo sapiens 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 the enzyme shows high substrate specificity for L-tyrosine Caenorhabditis elegans 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 the enzyme shows high substrate specificity for L-tyrosine Caenorhabditis elegans N2 Bristol 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 CAT-2
-
 Caenorhabditis elegans
1.14.16.2 TH1
-
 Homo sapiens
1.14.16.2 tyrosine hydroxylase
-
 Homo sapiens
1.14.16.2 tyrosine hydroxylase
-
 Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.2 tetrahydrobiopterin
-
 Homo sapiens
1.14.16.2 tetrahydrobiopterin
-
 Caenorhabditis elegans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.16.2 0.0022
-
 at pH 7.0 and 25°C Homo sapiens 3,4-dihydroxy-L-phenylalanine
1.14.16.2 0.0326
-
 at pH 7.0 and 25°C Caenorhabditis elegans 3,4-dihydroxy-L-phenylalanine

General Information

EC Number General Information Comment Organism
1.14.16.2 metabolism tyrosine hydroxylase is the key enzyme in the synthesis of dopamine Homo sapiens
1.14.16.2 metabolism tyrosine hydroxylase is the key enzyme in the synthesis of dopamine Caenorhabditis elegans