Data extracted from this reference:
Inhibitors
2.4.99.20
Cu2+
Oryctolagus cuniculus
2.4.99.20
additional information
the cyclization reaction is insensitive to free Ca2+ concentration in the range 20 nM–100 microM. Mg2+ is not inhibitory
Oryctolagus cuniculus
2.4.99.20
NAD+
Oryctolagus cuniculus
2.4.99.20
NADP+
Oryctolagus cuniculus
2.4.99.20
nicotinamide
inhibition of the cyclization reaction and is 30fold more potent at suppressing the base-exchange reaction. Inhibition is not via a competition with the nicotinic acid-binding site
Oryctolagus cuniculus
2.4.99.20
Zn2+
Oryctolagus cuniculus
KM Value [mM]
2.4.99.20
0.0334
NGDP+
pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Localization
2.4.99.20
membrane
of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16020
2.4.99.20
sarcoplasmic reticulum
membrane of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16529
Organism
2.4.99.20
Oryctolagus cuniculus
skeletal muscle isoform
Substrates and Products (Substrate)
2.4.99.20
2'-phospho-cyclic ADP-ribose + nicotinate
726898
Oryctolagus cuniculus
nicotinate-adenine dinucleotide phosphate
?
2.4.99.20
additional information
the cyclization reaction to form cADPr and cGDPr as well as the base-exchange reaction to form nicotinic acid adenine dinucleotide phosphate are strictly dependent on pH. Although the formation of cyclic GDP-ribose is optimized at pH 6, the synthesis of nicotinic acid adenine dinucleotide phosphate is most pronounced at a pH below 5. Nicotinic acid has virtually no influence on the cyclization reaction, but increases the affinity of NADP at an acidic pH and has the opposite effect at alkaline pH
726898
Oryctolagus cuniculus
?
2.4.99.20
NADP+
726898
Oryctolagus cuniculus
2'-phospho-cyclic ADP-ribose + nicotinamide
?
2.4.99.20
NGDP+
726898
Oryctolagus cuniculus
2'-phospho-cyclic GDP-ribose + nicotinamide
?
IC50 Value
2.4.99.20
0.00027
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Cu2+
2.4.99.20
0.0013
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NADP+
2.4.99.20
0.0023
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Zn2+
2.4.99.20
0.0054
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NAD+
2.4.99.20
0.7
cyclization reaction, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
nicotinamide
IC50 Value (protein specific)
2.4.99.20
0.00027
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Cu2+
2.4.99.20
0.0013
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NADP+
2.4.99.20
0.0023
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Zn2+
2.4.99.20
0.0054
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NAD+
2.4.99.20
0.7
cyclization reaction, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
nicotinamide
Inhibitors (protein specific)
2.4.99.20
Cu2+
Oryctolagus cuniculus
2.4.99.20
additional information
the cyclization reaction is insensitive to free Ca2+ concentration in the range 20 nM–100 microM. Mg2+ is not inhibitory
Oryctolagus cuniculus
2.4.99.20
NAD+
Oryctolagus cuniculus
2.4.99.20
NADP+
Oryctolagus cuniculus
2.4.99.20
nicotinamide
inhibition of the cyclization reaction and is 30fold more potent at suppressing the base-exchange reaction. Inhibition is not via a competition with the nicotinic acid-binding site
Oryctolagus cuniculus
2.4.99.20
Zn2+
Oryctolagus cuniculus
KM Value [mM] (protein specific)
2.4.99.20
0.0334
NGDP+
pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Localization (protein specific)
2.4.99.20
membrane
of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16020
2.4.99.20
sarcoplasmic reticulum
membrane of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16529
Substrates and Products (Substrate) (protein specific)
2.4.99.20
2'-phospho-cyclic ADP-ribose + nicotinate
726898
Oryctolagus cuniculus
nicotinate-adenine dinucleotide phosphate
?
2.4.99.20
additional information
the cyclization reaction to form cADPr and cGDPr as well as the base-exchange reaction to form nicotinic acid adenine dinucleotide phosphate are strictly dependent on pH. Although the formation of cyclic GDP-ribose is optimized at pH 6, the synthesis of nicotinic acid adenine dinucleotide phosphate is most pronounced at a pH below 5. Nicotinic acid has virtually no influence on the cyclization reaction, but increases the affinity of NADP at an acidic pH and has the opposite effect at alkaline pH
726898
Oryctolagus cuniculus
?
2.4.99.20
NADP+
726898
Oryctolagus cuniculus
2'-phospho-cyclic ADP-ribose + nicotinamide
?
2.4.99.20
NGDP+
726898
Oryctolagus cuniculus
2'-phospho-cyclic GDP-ribose + nicotinamide
?