BRENDA - Enzyme Database show

Channelling of substrate promiscuity of the skeletal-muscle ADP-ribosyl cyclase isoform

Bacher, I.; Zidar, A.; Kratzel, M.; Hohenegger, M.; Biochem. J. 381, 147-154 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.99.20
Cu2+
-
Oryctolagus cuniculus
2.4.99.20
additional information
the cyclization reaction is insensitive to free Ca2+ concentration in the range 20 nM–100 microM. Mg2+ is not inhibitory
Oryctolagus cuniculus
2.4.99.20
NAD+
-
Oryctolagus cuniculus
2.4.99.20
NADP+
-
Oryctolagus cuniculus
2.4.99.20
nicotinamide
inhibition of the cyclization reaction and is 30fold more potent at suppressing the base-exchange reaction. Inhibition is not via a competition with the nicotinic acid-binding site
Oryctolagus cuniculus
2.4.99.20
Zn2+
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.99.20
0.0334
-
NGDP+
pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.99.20
membrane
of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16020
-
2.4.99.20
sarcoplasmic reticulum
membrane of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16529
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.99.20
Oryctolagus cuniculus
-
skeletal muscle isoform
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.99.20
2'-phospho-cyclic ADP-ribose + nicotinate
-
726898
Oryctolagus cuniculus
nicotinate-adenine dinucleotide phosphate
-
-
-
?
2.4.99.20
additional information
the cyclization reaction to form cADPr and cGDPr as well as the base-exchange reaction to form nicotinic acid adenine dinucleotide phosphate are strictly dependent on pH. Although the formation of cyclic GDP-ribose is optimized at pH 6, the synthesis of nicotinic acid adenine dinucleotide phosphate is most pronounced at a pH below 5. Nicotinic acid has virtually no influence on the cyclization reaction, but increases the affinity of NADP at an acidic pH and has the opposite effect at alkaline pH
726898
Oryctolagus cuniculus
?
-
-
-
-
2.4.99.20
NADP+
-
726898
Oryctolagus cuniculus
2'-phospho-cyclic ADP-ribose + nicotinamide
-
-
-
?
2.4.99.20
NGDP+
-
726898
Oryctolagus cuniculus
2'-phospho-cyclic GDP-ribose + nicotinamide
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.4.99.20
0.00027
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Cu2+
2.4.99.20
0.0013
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NADP+
2.4.99.20
0.0023
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Zn2+
2.4.99.20
0.0054
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NAD+
2.4.99.20
0.7
-
cyclization reaction, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
nicotinamide
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.4.99.20
0.00027
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Cu2+
2.4.99.20
0.0013
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NADP+
2.4.99.20
0.0023
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Zn2+
2.4.99.20
0.0054
-
formation of cGDPr, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
NAD+
2.4.99.20
0.7
-
cyclization reaction, pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
nicotinamide
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.99.20
Cu2+
-
Oryctolagus cuniculus
2.4.99.20
additional information
the cyclization reaction is insensitive to free Ca2+ concentration in the range 20 nM–100 microM. Mg2+ is not inhibitory
Oryctolagus cuniculus
2.4.99.20
NAD+
-
Oryctolagus cuniculus
2.4.99.20
NADP+
-
Oryctolagus cuniculus
2.4.99.20
nicotinamide
inhibition of the cyclization reaction and is 30fold more potent at suppressing the base-exchange reaction. Inhibition is not via a competition with the nicotinic acid-binding site
Oryctolagus cuniculus
2.4.99.20
Zn2+
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.99.20
0.0334
-
NGDP+
pH 7.4, temperature not specified in the publication
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.99.20
membrane
of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16020
-
2.4.99.20
sarcoplasmic reticulum
membrane of heavy sarcoplasmic reticulum
Oryctolagus cuniculus
16529
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.99.20
2'-phospho-cyclic ADP-ribose + nicotinate
-
726898
Oryctolagus cuniculus
nicotinate-adenine dinucleotide phosphate
-
-
-
?
2.4.99.20
additional information
the cyclization reaction to form cADPr and cGDPr as well as the base-exchange reaction to form nicotinic acid adenine dinucleotide phosphate are strictly dependent on pH. Although the formation of cyclic GDP-ribose is optimized at pH 6, the synthesis of nicotinic acid adenine dinucleotide phosphate is most pronounced at a pH below 5. Nicotinic acid has virtually no influence on the cyclization reaction, but increases the affinity of NADP at an acidic pH and has the opposite effect at alkaline pH
726898
Oryctolagus cuniculus
?
-
-
-
-
2.4.99.20
NADP+
-
726898
Oryctolagus cuniculus
2'-phospho-cyclic ADP-ribose + nicotinamide
-
-
-
?
2.4.99.20
NGDP+
-
726898
Oryctolagus cuniculus
2'-phospho-cyclic GDP-ribose + nicotinamide
-
-
-
?