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Literature summary extracted from

  • Morii, H.; Ogawa, M.; Fukuda, K.; Taniguchi, H.
    Ubiquitous distribution of phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase in bacteria and archaea, which contain inositol phospholipid (2013), Biochem. Biophys. Res. Commun., 443, 86-90.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.39 expression in Escherichia coli Aeropyrum pernix
2.7.8.39 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.11 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Homo sapiens cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 7 pmol/h/mg and 2 pmol/h/mg, respectively Homo sapiens
2.7.8.11 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Saccharomyces cerevisiae cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 107 pmol/h/mg and 7 pmol/h/mg, respectively Saccharomyces cerevisiae
2.7.8.B13 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Corynebacterium glutamicum cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 175 pmol/h/mg and 174 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview Corynebacterium glutamicum
2.7.8.B13 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Mycobacterium bovis cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 138 pmol/h/mg and 5 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview Mycobacterium tuberculosis variant bovis
2.7.8.B13 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Propionibacterium acnes cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 88 pmol/h/mg and 14 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview Cutibacterium acnes
2.7.8.B13 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Rhodococcus equi cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 124 pmol/h/mg and 33 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview Rhodococcus equi
2.7.8.B13 additional information an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Streptomyces avermitilis cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 76 pmol/h/mg and 20 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview Streptomyces avermitilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.11 Mn2+ required Homo sapiens
2.7.8.11 Mn2+ required Saccharomyces cerevisiae
2.7.8.B13 Mg2+ required Corynebacterium glutamicum
2.7.8.B13 Mg2+ required Rhodococcus equi
2.7.8.B13 Mg2+ required Cutibacterium acnes
2.7.8.B13 Mg2+ required Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol Homo sapiens
-
 CMP + 1-phosphatidyl-1D-myo-inositol
-
 ?
2.7.8.11 CDP-diacylglycerol + myo-inositol Saccharomyces cerevisiae
-
 CMP + 1-phosphatidyl-1D-myo-inositol
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate Corynebacterium glutamicum specificity of PIP synthase for lipid substrates CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate Rhodococcus equi specificity of PIP synthase for lipid substrates CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate Mycobacterium tuberculosis variant bovis specificity of PIP synthase for lipid substrates CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate Cutibacterium acnes specificity of PIP synthase for lipid substrates CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate Streptomyces avermitilis specificity of PIP synthase for lipid substrates CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.11 Homo sapiens
-
-
-
2.7.8.11 Saccharomyces cerevisiae P70500
-
-
2.7.8.B13 Corynebacterium glutamicum
-
-
-
2.7.8.B13 Cutibacterium acnes
-
-
-
2.7.8.B13 Mycobacterium tuberculosis variant bovis
-
-
-
2.7.8.B13 Rhodococcus equi
-
-
-
2.7.8.B13 Streptomyces avermitilis
-
-
-
2.7.8.39 Aeropyrum pernix Q9YBS3
-
-
2.7.8.39 Aeropyrum pernix DSM 11879 Q9YBS3
-
-
2.7.8.39 Saccharolobus solfataricus Q7LXU6
-
-
2.7.8.39 Saccharolobus solfataricus P2 Q7LXU6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.39 1783
-
 pH 6.0, 80°C Saccharolobus solfataricus
2.7.8.39 1867
-
 pH 6.0, 80°C Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol
-
 Homo sapiens CMP + 1-phosphatidyl-1D-myo-inositol
-
 ?
2.7.8.11 CDP-diacylglycerol + myo-inositol
-
 Saccharomyces cerevisiae CMP + 1-phosphatidyl-1D-myo-inositol
-
 ?
2.7.8.11 CDP-diacylglycerol + myo-inositol specificity of PI synthase for the substrates, no activity with 1L-myo-inositol 1-phosphate Homo sapiens CMP + 1-phosphatidyl-1D-myo-inositol
-
 ?
2.7.8.11 CDP-diacylglycerol + myo-inositol specificity of PI synthase for the substrates, no activity with 1L-myo-inositol 1-phosphate Saccharomyces cerevisiae CMP + 1-phosphatidyl-1D-myo-inositol
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate
-
 Corynebacterium glutamicum CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate
-
 Rhodococcus equi CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate
-
 Mycobacterium tuberculosis variant bovis CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate
-
 Cutibacterium acnes CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate
-
 Streptomyces avermitilis CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate specificity of PIP synthase for lipid substrates Corynebacterium glutamicum CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate specificity of PIP synthase for lipid substrates Rhodococcus equi CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate specificity of PIP synthase for lipid substrates Mycobacterium tuberculosis variant bovis CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate specificity of PIP synthase for lipid substrates Cutibacterium acnes CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.B13 CDP-diacylglycerol + 1L-myo-inositol 1-phosphate specificity of PIP synthase for lipid substrates Streptomyces avermitilis CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.39 CDP-2,3-bis-(O-phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
 Aeropyrum pernix CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.39 CDP-2,3-bis-(O-phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
 Saccharolobus solfataricus CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.39 CDP-2,3-bis-(O-phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
 Aeropyrum pernix DSM 11879 CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.39 CDP-2,3-bis-(O-phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
 Saccharolobus solfataricus P2 CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.11 phosphatidylinositol synthase
-
 Homo sapiens
2.7.8.11 phosphatidylinositol synthase
-
 Saccharomyces cerevisiae
2.7.8.11 PI synthase
-
 Homo sapiens
2.7.8.11 PI synthase
-
 Saccharomyces cerevisiae
2.7.8.B13 phosphatidylinositol phosphate synthase
-
 Corynebacterium glutamicum
2.7.8.B13 phosphatidylinositol phosphate synthase
-
 Rhodococcus equi
2.7.8.B13 phosphatidylinositol phosphate synthase
-
 Mycobacterium tuberculosis variant bovis
2.7.8.B13 phosphatidylinositol phosphate synthase
-
 Cutibacterium acnes
2.7.8.B13 phosphatidylinositol phosphate synthase
-
 Streptomyces avermitilis
2.7.8.B13 PIP synthase
-
 Corynebacterium glutamicum
2.7.8.B13 PIP synthase
-
 Rhodococcus equi
2.7.8.B13 PIP synthase
-
 Mycobacterium tuberculosis variant bovis
2.7.8.B13 PIP synthase
-
 Cutibacterium acnes
2.7.8.B13 PIP synthase
-
 Streptomyces avermitilis
2.7.8.39 AIP synthase
-
 Aeropyrum pernix
2.7.8.39 AIP synthase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.11 30
-
 assay at Saccharomyces cerevisiae
2.7.8.11 37
-
 assay at Homo sapiens
2.7.8.B13 37
-
 assay at Corynebacterium glutamicum
2.7.8.B13 37
-
 assay at Rhodococcus equi
2.7.8.B13 37
-
 assay at Cutibacterium acnes
2.7.8.B13 37
-
 assay at Streptomyces avermitilis
2.7.8.39 80
-
 assay at Aeropyrum pernix
2.7.8.39 80
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.11 8
-
 assay at Homo sapiens
2.7.8.11 8
-
 assay at Saccharomyces cerevisiae
2.7.8.B13 8
-
 assay at Corynebacterium glutamicum
2.7.8.B13 8
-
 assay at Rhodococcus equi
2.7.8.B13 8
-
 assay at Cutibacterium acnes
2.7.8.B13 8
-
 assay at Streptomyces avermitilis
2.7.8.39 6
-
 assay at Aeropyrum pernix
2.7.8.39 6
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
2.7.8.11 metabolism in eukarya, phosphatidylinositol (PI) is only biosynthesized from CDP-diacylglycerol (CDP-DAG) and inositol Homo sapiens
2.7.8.11 metabolism in eukarya, phosphatidylinositol (PI) is only biosynthesized from CDP-diacylglycerol (CDP-DAG) and inositol Saccharomyces cerevisiae
2.7.8.B13 metabolism in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid Corynebacterium glutamicum
2.7.8.B13 metabolism in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid Rhodococcus equi
2.7.8.B13 metabolism in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid Mycobacterium tuberculosis variant bovis
2.7.8.B13 metabolism in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid Cutibacterium acnes
2.7.8.B13 metabolism in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid Streptomyces avermitilis