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Literature summary extracted from

  • Inouye, S.; Sato, J.; Sahara-Miura, Y.; Yoshida, S.; Kurakata, H.; Hosoya, T.
    C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: the mutated catalytic 19 kDa component of Oplophorus luciferase (2013), Biochem. Biophys. Res. Commun., 437, 23-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.13 the fused proteinof nanoKAZ with IgG-binding domain (ZZ domain) is expressed in the cytoplasm of Escherichia coli cells as a soluble form and purified. A secretory expression of nanoKAZ in CHO-K1 cells is performed using nanoKAZ fused with the signal peptide sequence of Gaussia luciferase Oplophorus gracilirostris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.13 20000
-
 Western blot, expression in CHO cells Oplophorus gracilirostris

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.13 Oplophorus gracilirostris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.13 using Ni-NTA chromatography Oplophorus gracilirostris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.13 6h-coelenterazine + O2 luminescence intensity (Imax): 0.8 Oplophorus gracilirostris oxidized 6h-coelenterazine + CO2 + hnu
-
 ?
1.13.12.13 6h-f-coelenterazine + O2 luminescence intensity (Imax): 10.1 Oplophorus gracilirostris oxidized 6h-f-coelenterazine + CO2 + hnu
-
 ?
1.13.12.13 bis-coelenterazine + O2 luminescence intensity (Imax): 10.3 Oplophorus gracilirostris oxidized bis-coelenterazine + CO2 + hnu
-
 ?
1.13.12.13 coelenterazine + O2 luminescence intensity (Imax): 1.0 Oplophorus gracilirostris oxidized coelenterazine + CO2 + hn
-
 ?
1.13.12.13 f-coelenterazine + O2 luminescence intensity (Imax): 19.5 Oplophorus gracilirostris oxidized f-coelenterazine + CO2 + hnu
-
 ?
1.13.12.13 furimazine + O2 luminescence intensity (Imax): 10.1 Oplophorus gracilirostris ?
-
 ?
1.13.12.13 h-coelenterazine + O2 luminescence intensity (Imax): 17 Oplophorus gracilirostris oxidized h-coelenterazine + CO2 + hnu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.13 nanoKAZ mutated catalytic component of Oplophorus luciferase Oplophorus gracilirostris
1.13.12.13 Oplophorus luciferase
-
 Oplophorus gracilirostris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.13 7.6
-
 assay at Oplophorus gracilirostris