BRENDA - Enzyme Database show

Human holocarboxylase synthetase with a start site at methionine-58 is the predominant nuclear variant of this protein and has catalytic activity

Bao, B.; Wijeratne, S.S.; Rodriguez-Melendez, R.; Zempleni, J.; Biochem. Biophys. Res. Commun. 412, 115-120 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.3.4.10
expressed in HEK-293 cells
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.4.10
cytoplasm
localizes predominantly in the cytoplasm
Homo sapiens
5737
-
6.3.4.10
nucleus
-
Homo sapiens
5634
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.10
115000
-
N-terminal glutathione S transferase-tagged enzyme, x * 115000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.4.10
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
Homo sapiens
-
AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.4.10
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.10
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
726859
Homo sapiens
AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.4.10
?
N-terminal glutathione S transferase-tagged enzyme, x * 115000, SDS-PAGE
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.3.4.10
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.10
expressed in HEK-293 cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.3.4.10
ATP
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.4.10
cytoplasm
localizes predominantly in the cytoplasm
Homo sapiens
5737
-
6.3.4.10
nucleus
-
Homo sapiens
5634
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.10
115000
-
N-terminal glutathione S transferase-tagged enzyme, x * 115000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.4.10
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
Homo sapiens
-
AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.10
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
726859
Homo sapiens
AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.4.10
?
N-terminal glutathione S transferase-tagged enzyme, x * 115000, SDS-PAGE
Homo sapiens