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Literature summary extracted from

  • Hatakeyama, S.; Nakayama, K.I.
    U-box proteins as a new family of ubiquitin ligases (2003), Biochem. Biophys. Res. Commun., 302, 635-645.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.B12 expression in HeLa cell, Escherichia coli Homo sapiens
2.3.2.B12 expression in HeLa cell, Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.B12 additional information deletion of the U-box domain abolishes E3 activity of isoform UFD2a Mus musculus
2.3.2.B12 additional information isoform CHIP mutants lacking the U box domain fail to exhibit polyubiquitination activity Mus musculus
2.3.2.B12 P1140A mutation of the conserved proline residue, abolishes E3 activity of isoform UFD2a Mus musculus
2.3.2.B12 P270A loss of polyubiquitination activity Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.B12 cytoplasm
-
 Mus musculus 5737
-
2.3.2.B12 cytoplasm
-
 Homo sapiens 5737
-
2.3.2.B12 nucleus
-
 Mus musculus 5634
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.B12 Homo sapiens HSU37219 isoform CYC4
-
2.3.2.B12 Homo sapiens XM006045 isoform Prp19
-
2.3.2.B12 Mus musculus Q9WUD1 isoform CHIP
-
2.3.2.B12 Mus musculus Q9ES00 isoform UFD2a
-
2.3.2.B12 Mus musculus AI390103 isoform UFD2b
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.B12 brain
-
 Mus musculus
-
2.3.2.B12 brain
-
 Homo sapiens
-
2.3.2.B12 heart
-
 Mus musculus
-
2.3.2.B12 heart
-
 Homo sapiens
-
2.3.2.B12 kidney
-
 Mus musculus
-
2.3.2.B12 kidney
-
 Homo sapiens
-
2.3.2.B12 liver
-
 Mus musculus
-
2.3.2.B12 pancreas
-
 Homo sapiens
-
2.3.2.B12 placenta
-
 Homo sapiens
-
2.3.2.B12 skeletal muscle
-
 Homo sapiens
-
2.3.2.B12 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.B12 additional information data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3 Mus musculus ?
-
 ?
2.3.2.B12 additional information data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity. enzyme is able to catalyze ubiquitin chain formation on artificial substrates in the presence of E1 and E2 and in the absence of E3 Homo sapiens ?
-
 ?
2.3.2.B12 [CHIP U-box-E4-ubiquitin-carrier protein Ubc4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CHIP is greatest with E2 enzymes Ubc4 or UbcH5C Mus musculus [CHIP U-box-E4-ubiquitin-carrier protein Ubc4]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [CHIP U-box-E4-ubiquitin-carrier protein UbH5C4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CHIP is greatest with E2 enzymes Ubc4 or UbcH5C Mus musculus [CHIP U-box-E4-ubiquitin-carrier protein UbcH5C]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [CYC4 U-box-E4-ubiquitin-carrier protein Ubc2B]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CYC4 is greatest with E2 enzymes Ubc2B or Ubc3, but is also activ with UbcH7 Homo sapiens [CYC4 U-box-E4-ubiquitin-carrier protein Ubc2B]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [CYC4 U-box-E4-ubiquitin-carrier protein Ubc3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CYC4 is greatest with E2 enzymes Ubc2 or Ubc3, but is also activ with UbcH7 Homo sapiens [CYC4 U-box-E4-ubiquitin-carrier protein Ubc3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [CYC4 U-box-E4-ubiquitin-carrier protein UbcH7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of CYC4 is greatest with E2 enzymes Ubc2 or Ubc3, but is also activ with UbcH7 Homo sapiens [CYC4 U-box-E4-ubiquitin-carrier protein UbcH7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [PRP19 U-box-E4-ubiquitin-carrier protein Ubc3]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of PRP19 is greatest with E2 enzyme Ubc3 Homo sapiens [PRP19 U-box-E4-ubiquitin-carrier protein Ubc3]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [UFD2a U-box-E4-ubiquitin-carrier protein Ubc4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of UFD2a is greatest with E2 enzymes Ubc4 or UbcH5C Mus musculus [U-box-E4-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [UFD2b U-box-E4-ubiquitin-carrier protein Ubc4]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of UFD2b is greatest with E2 enzymes Ubc4 or UbcH5C Mus musculus [UFD2b U-box-E4-ubiquitin-carrier protein Ubc4]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?
2.3.2.B12 [UFD2b U-box-E4-ubiquitin-carrier protein UbH5C]-S-ubiquitinyl-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine enzyme mediates polyubiquitination in the presence of E1 and E2 and in the absence of E3, exhibiting different specificities for E2 enzymes. Ubiquination activity of UFD2b is greatest with E2 enzymes Ubc4 or UbcH5C Mus musculus [UFD2b U-box-E4-ubiquitin-carrier protein UbcH5C]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-ubiquitinyl-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.B12 CHIP
-
 Mus musculus
2.3.2.B12 CYC4
-
 Homo sapiens
2.3.2.B12 PRP19
-
 Homo sapiens
2.3.2.B12 ubiquitin fusion degradation protein 2
-
 Mus musculus
2.3.2.B12 Ufd2a
-
 Mus musculus
2.3.2.B12 Ufd2b
-
 Mus musculus